Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer
Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer
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DOI：
10.1016/s0020-7519(01)00357-5
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发表时间：
2002-05-01
影响因子：
4
通讯作者：
Blouin, MS
中科院分区：
文献类型：
--
作者：
Blouin, MS
比较了线虫内部转录间隔区（ITS-1和ITS-2）与线粒体coxl或nad4位点的DNA序列差异。线粒体序列积累取代比内部转录间隔更快，这种差异在最密切相关的物种对中最为显著。因此，线粒体DNA可能是使用少量个体的序列数据来搜索潜在的隐物种的最佳选择。另一方面，由于其较低的种内多态性水平，内部转录间隔序列仍然是DNA诊断（快速区分已知物种）的优秀工具。(C) 2002澳大利亚寄生虫学学会Elsevier Science Ltd.出版。版权所有。
DNA sequence divergence at internal transcribed spacer regions (ITS-1 and ITS-2) was compared with divergence at mitochondrial coxl or nad4 loci in pairs of congeneric nematode species. Mitochondrial sequences accumulate substitutions much more quickly than internal transcribed spacer, the difference being most striking in the most closely related species pairs. Thus, mitochondrial DNA may be the best choice for applications in which one is using sequence data on small numbers of individuals to search for potential cryptic species. On the other hand, internal transcribed spacer remains an excellent tool for DNA diagnostics (quickly distinguishing between known species) owing to its lower level of intraspecific polymorphism. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.