Molecular evidence of natural hybridization between Fasciola hepatica and F-gigantica
Molecular evidence of natural hybridization between Fasciola hepatica and F-gigantica
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DOI：
10.1016/s1383-5769(00)00051-9
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发表时间：
2000-09-01
影响因子：
1.9
通讯作者：
Hong, SJ
中科院分区：
文献类型：
--
作者：
Agatsuma, T;Arakawa, Y;Hong, SJ
为了阐明片形吸虫属（Fasciola）的分类地位，对5种朝鲜蠕虫的线粒体DNA的细胞色素c氧化酶亚基1（CO 1）和NADH脱氢酶亚基1（ND 1）以及核DNA的28 S rDNA（28 S）的内转录间隔区2（ITS 2）和D2区进行了核苷酸序列测定。朝鲜蠕虫的CO 1和ND 1区域均为单态，与F.南极洲另一方面，ITS 2和D2区域被发现是多态的;也就是说，在五个蠕虫中，两个具有F。南极型层序1个，F.肝型序列和两个拥有这两种类型的序列，表明存在不同的等位基因的基因座。应该注意的是，ITS 2和D2区域的这些变异同时发生在同一个体蠕虫上。通过对每种蠕虫的5至6个克隆PCR产物进行测序证实了这一点。本研究强烈建议种间杂交的两个物种共存于韩国。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Nucleotide sequences of two regions, cytochrome c-oxidase subunit 1 (CO1) and NADH dehydrogenase subunit 1 (ND1) of the mitochondrial DNA and two regions, internal-transcribed spacer 2 (ITS2) and the D2 region in the 28S rDNA (28S) of the nuclear DNA were obtained from five Korean worms of the genus Fasciola in order to elucidate their taxonomic status. The CO1 and ND1 regions are all monomorphic in the Korean worms and similar to those of F. gigantica. On the other hand, the ITS2 and D2 regions were found to be polymorphic; that is, out of five worms, two possessed a F. gigantica-type sequence, one, a F. hepatica-type sequence and two possessed sequences of both types indicating an existence of different alleles at the loci. It should be noted that these Variations of the ITS2 and D2 regions co-occur at the same individual worms. This was confirmed by sequencing five to six cloned PCR products for each worm. The present study strongly suggests interspecific cross-hybridization between the two species coexisting in Korea. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.