Determination of uric acid in human saliva by capillary electrophoresis with electrochemical detection: potential application in fast diagnosis of gout
Determination of uric acid in human saliva by capillary electrophoresis with electrochemical detection: potential application in fast diagnosis of gout
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DOI：
10.1007/s00216-004-2848-y
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发表时间：
2004-12-01
影响因子：
4.3
通讯作者：
Ye, JN
中科院分区：
文献类型：
--
作者：
Guan, YQ;Chu, QC;Ye, JN
痛风的临床表现是尿酸(UA)晶体形成和沉积的结果。对于痛风、高尿酸血症和Lesch-Nyhan综合征的诊断和治疗，建议监测唾液等侵入性较小的生物标本中的尿酸水平。为了研究人唾液和尿液中尿酸的相关性，探索其在痛风快速诊断中的潜在应用价值，采用毛细管电泳电泳法(CE-ED)测定人唾液和尿液中的尿酸。在最佳分离条件下，分离电压为14kV，在80 mmol L-1硼砂缓冲液(pH 7.8)中，尿酸与3种共存物质在14min内即可得到很好的分离。峰电流与分析物浓度在两个数量级内呈良好的线性关系，检出限(S/N=3)为1.09×10~(-7)~5.0×10~(-7)mol L~(-1)。该方法已成功地用于研究人唾液和尿液中尿酸含量的相关性，为痛风的快速诊断提供了一种新的、方便的方法。
The clinical manifestations of gout result from the formation and deposition of uric acid (UA) crystals. The monitoring of, UA level in less invasive biological samples such as saliva is suggested for diagnosis and therapy of gout, hyperuricemia and the Lesch-Nyhan syndrome. In order to investigate the correlation between trace amounts of UA in human saliva and urine and explore the potential application in fast diagnosis of gout, capillary electrophoresis with electrochemical detection (CE-ED) was applied for the determination of UA in human saliva and urine in this work. Under the optimum conditions, UA and three coexisting analytes could be well separated within 14 min at the separation voltage of 14 kV in 80 mmol L-1 borax running buffer (pH 7.8). A good linear relationship was established between peak current and concentration of analytes over two orders of magnitude with detection limits (S/N = 3) ranging from 1.09 x 10(-7) to 5.0 x 10(-7) mol L-1 for all analytes. This proposed method has been successfully applied for study of the correlation between the UA content-of human saliva and urine, providing an alternative and convenient method for rapid diagnosis of gout.