The role of haemagglutinin-neuraminidase glycoprotein cell surface expression in the survival of Sendai virus-infected BHK-21 cells.
The role of haemagglutinin-neuraminidase glycoprotein cell surface expression in the survival of Sendai virus-infected BHK-21 cells.
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血凝素神经氨酸酶糖蛋白细胞表面表达在仙台病毒感染的 BHK-21 细胞存活中的作用。
DOI：
10.1099/0022-1317-66-11-2313
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Roux,L
中科院分区：
文献类型：
--
作者：
Tuffereau,C;Portner,A;Roux,L
利用带有血凝素神经氨酸酶蛋白(HN)损伤的仙台病毒温度敏感突变体(Ts271)，研究了HN细胞表面表达对感染BHK-21细胞命运的影响。在不允许的温度(38摄氏度)下，HN的总量被减少到271个感染病毒的细胞中，可能是由于蛋白质的降解。在此温度下，感染细胞表面既没有表达HN，也没有修饰形式的HN。然而，感染了271的BHK-21细胞在38℃和30℃时仍被感染而死亡。这些结果排除了HN细胞表面表达的缺乏可能是BHK细胞存活的唯一要求的假设。
A temperature-sensitive mutant of Sendai virus with a lesion in the haemagglutinin-neuraminidase protein (HN) (ts271) was used to study the effect of HN cell surface expression on the fate of infected BHK-21 cells. The total amount of HN was reduced ints271 virus-infected cells at the non-permissive temperature (38 °C) presumably due to degradation of the protein. At this temperature, neither HN nor a modified form of HN were found expressed at the surface of the infected cells. BHK-21 cells infected withts271 were nevertheless killed by the infection at 38 °C as well as at 30 °C. These results ruled out the hypothesis that the lack of HN cell surface expression could be the unique requirement allowing BHK cell survival.