Molecular Epidemiology of Plum pox virus in Japan
Molecular Epidemiology of Plum pox virus in Japan
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DOI：
10.1094/phyto-10-10-0280
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发表时间：
2011-05-01
期刊：
影响因子：
3.2
通讯作者：
Namba, Shigetou
中科院分区：
文献类型：
--
作者：
Maejima, Kensaku;Himeno, Misako;Namba, Shigetou
为了进行基于完整基因组序列的分子流行病学研究，从日本关东地区收集了 37 株李痘病毒 (PPV) 分离株。配对分析显示，所有 37 个日本分离株均属于 PPV-D 株，遗传多样性较低（小于 0.8%）。在基于完整核苷酸序列的PPV-D毒株的系统发育分析中，高分辨率地重建了PPV-D毒株的关系：在全球水平上，美国、加拿大和日本的分离株形成了各自独特的单系群，表明病毒进入这些国家的途径是独立的；在地方层面，PPV 的实际传播历史在高 bootstrap 支持下被精确重建。这是首次基于完整的侏儒序列对 PPV 的分子流行病学进行描述。
For a molecular epidemiological study based on complete genome sequences, 37 Plum pox virus (PPV) isolates were collected from the Kanto region in Japan. Pair-wise analyses revealed that all 37 Japanese isolates belong to the PPV-D strain, with low genetic diversity (less than 0.8%). In phylogenetic analysis of the PPV-D strain based on complete nucleotide sequences, the relationships of the PPV-D strain were reconstructed with high resolution: at the global level, the American, Canadian, and Japanese isolates formed their own distinct monophyletic clusters, suggesting that the routes of viral entry into these countries were independent; at the local level, the actual transmission histories of PPV were precisely reconstructed with high bootstrap support. This is the first description of the molecular epidemiology of PPV based on complete gnome sequences.