Keratin 17 testing in pancreatic cancer needle aspiration biopsies predicts survival.
Keratin 17 testing in pancreatic cancer needle aspiration biopsies predicts survival.
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DOI：
10.1002/cncy.22438
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发表时间：
2021-11
影响因子：
3.4
通讯作者：
Shroyer, Kenneth R.
中科院分区：
文献类型：
--
作者：
Roa-Pena, Lucia;Babu, Sruthi;Leiton, Cindy V.;Wu, Maoxin;Taboada, Sofia;Akalin, Ali;Buscaglia, Jonathan;Escobar-Hoyos, Luisa F.;Shroyer, Kenneth R.
关键词：
尽管胰腺导管腺癌（pancreatic ductal adencarcinoma， PDAC）是所有癌症中5年生存率最低的癌症之一，但具有相同临床特征的患者之间的生存率存在差异。作者先前证明，角蛋白17 （K17）在PDAC中的表达，通过RNA测序或免疫组织化学（IHC）测量，是一个独立的阴性预后生物标志物。只有20%的病例适合手术切除，但大多数患者是通过针吸活检（NAB）诊断的。本研究的目的是确定匹配NAB中检测到的K17评分与手术切除组织切片是否存在相关性，以及NAB细胞块标本中的K17免疫组化是否可以作为PDAC的阴性预后生物标志物。对70例NAB细胞阻断和手术切除样本匹配的患者进行K17免疫组化，分析K17表达水平的相关性。K17免疫组化也在来自发现和验证队列的细胞块中进行。分析Kaplan-Meier和Cox比例风险回归模型，确定不同K17 IHC表达水平病例的生存差异。K17 IHC在匹配的nab和切除组织中表达相关。当≥80%的肿瘤细胞显示强（2+）染色时，NAB样品被归类为高K17水平。高k17病例，包括分期匹配的病例，生存时间较短。K17已被确定为一种强大且独立的预后生物标志物，可对NAB诊断病例的临床结果进行分层。K17的检测也有可能为优化化疗干预的临床决策提供信息。
Although pancreatic ductal adenocarcinoma (PDAC) has one of the lowest 5-year survival rates of all cancers, differences in survival exist between patients with clinically identical characteristics. The authors previously demonstrated that keratin 17 (K17) expression in PDAC, measured by RNA sequencing or immunohistochemistry (IHC), is an independent negative prognostic biomarker. Only 20% of cases are candidates for surgical resection, but most patients are diagnosed by needle aspiration biopsy (NAB). The aims of this study were to determine whether there was a correlation in K17 scores detected in matched NABs and surgical resection tissue sections and whether K17 IHC in NAB cell block specimens could be used as a negative prognostic biomarker in PDAC. K17 IHC was performed for a cohort of 70 patients who had matched NAB cell block and surgical resection samples to analyze the correlation of K17 expression levels. K17 IHC was also performed in cell blocks from discovery and validation cohorts. Kaplan-Meier and Cox proportional hazards regression models were analyzed to determine survival differences in cases with different levels of K17 IHC expression. K17 IHC expression correlated in matched NABs and resection tissues. NAB samples were classified as high for K17 when ≥80% of tumor cells showed strong (2+) staining. High-K17 cases, including stage-matched cases, had shorter survival. K17 has been identified as a robust and independent prognostic biomarker that stratifies clinical outcomes for cases that are diagnosed by NAB. Testing for K17 also has the potential to inform clinical decisions for optimization of chemotherapeutic interventions.
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DOI：
10.1038/modpathol.2013.166
发表时间：
2014-04
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.2
作者：
Escobar-Hoyos, Luisa F.;Shah, Ruchi;Shroyer, Kenneth R.
通讯作者：
Shroyer, Kenneth R.
影响因子：
7.5
作者：
Babu, Sruthi;Mockler, Daniel C.;Shroyer, Kenneth R.
通讯作者：
Shroyer, Kenneth R.
影响因子：
3.7
作者：
Kamarajah, Sivesh K.;Burns, William R.;Nathan, Hari
通讯作者：
Nathan, Hari
影响因子：
5.2
作者：
Kommalapati A;Tella SH;Goyal G;Ma WW;Mahipal A
通讯作者：
Mahipal A