Enhanced urinary excretion of leukotriene E4 by patients with multiple trauma with or without adult respiratory distress syndrome.
Enhanced urinary excretion of leukotriene E4 by patients with multiple trauma with or without adult respiratory distress syndrome.
复制标题
患有或不患有成人呼吸窘迫综合征的多发性创伤患者白三烯 E4 的尿排泄增强。
作者：
J. Fauler;D. Tsikas;M. Holch;A. Seekamp;M. Nerlich;J. Sturm;J. Frölich
1.本研究旨在探讨多发伤患者全身合成半胱氨基白三烯的情况。为了做到这一点，我们在创伤后的前10天评估了白三烯E4的尿液排泄量。2.用GC-MS确证了白三烯E4的结构。在健康受试者和多发性创伤患者的尿液中，它转化为5-羟基二十烷酸。白三烯E4常规通过固相萃取和反相高效毛细管电泳法从24小时尿样中分离出来。并随后被R.I.A量化。3.健康受试者每日尿中白三烯E4/肌酐排泄量为10+/-3nmoL(平均值+/-扫描电子显微镜，n=16)。4.无成人呼吸窘迫综合征的多发伤患者(n=7)在伤后前10天每天排出的白三烯E4/肌酐(平均+/-SEM)为76.8+/-6.7nmoL，显著高于正常对照组(P<0.01)。5.3例多发伤并发成人呼吸窘迫综合征患者白三烯E4排泄量增加。这三名患者在创伤后第9天最大排泄量为593+/-185nmol的白三烯E4/肌酐(平均值+/-扫描电子显微镜)，这相当于白三烯E4的排泄量分别是未发展为成人呼吸窘迫综合征的多发性创伤患者和健康受试者的7.7倍和59倍。
1. The aim of the present study was to evaluate the systemic synthesis of cysteinyl leukotrienes in patients with multiple trauma. In order to do this, the urinary excretion of leukotriene E4 was assessed in the first 10 days after trauma. 2. Leukotriene E4 was unequivocally identified by g.c.-m.s. in the urine of healthy subjects and patients with multiple trauma after its conversion to 5-hydroxyeicosanoic acid. Leukotriene E4 was routinely isolated from 24 h urine samples by solid-phase extraction followed by reverse-phase h.p.l.c. and was subsequently quantified by r.i.a. 3. Healthy subjects excreted daily 10 +/- 3 nmol of leukotriene E4/mol of creatinine (mean +/- SEM, n = 16) into urine. 4. Patients with multiple trauma who did not develop adult respiratory distress syndrome (n = 7) excreted 76.8 +/- 6.7 nmol of leukotriene E4/mol of creatinine (mean +/- SEM) daily during the first 10 days after trauma, which was significantly (P less than 0.01) more than did healthy subjects. 5. Excretion of leukotriene E4 was even more enhanced in three patients with multiple trauma who developed adult respiratory distress syndrome. Maximal amounts of 593 +/- 185 nmol of leukotriene E4/mol of creatinine (mean +/- SEM) were excreted on day 9 after trauma by these three patients, which corresponds to a 7.7- and a 59-fold increase in excretion of leukotriene E4 compared with patients with multiple trauma who did not develop adult respiratory distress syndrome and healthy subjects, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)