Analysis of high-mass biomolecules using electrostatic fields and matrix-assisted laser desorption/ionization in a Fourier transform mass spectrometer.
Analysis of high-mass biomolecules using electrostatic fields and matrix-assisted laser desorption/ionization in a Fourier transform mass spectrometer.
复制标题
在傅里叶变换质谱仪中使用静电场和基质辅助激光解吸/电离分析高质量生物分子。
DOI：
10.1021/ac00116a004
复制
发表时间：
1995
影响因子：
7.4
通讯作者：
Wilkins,CL
中科院分区：
文献类型：
--
作者：
Yao,J;Dey,M;Pastor,SJ;Wilkins,CL
一种新的减速技术，将直流电位上的正交激发和接收板，以及后捕获板（电导限制）的源细胞的双立方细胞已被应用到标准的基质辅助激光解吸/电离傅立叶变换质谱技术。当应用这种五板捕获方法时，可以有效地捕获和检测具有大平移动能的高质量离子。利用这种方法，低分辨率光谱碳酸酐酶（MW= 29 000），卵清蛋白（MW
A new decelerating technique that places dc potentials on the orthogonal excitation and receiver plates as well as the rear trapping plate (conductance limit) of the source cell of a dual cubic cell has been applied to the standard matrix-assisted laser desorption/ionization Fourier trans-form mass spectrometry technique. When this five-plate trapping method is applied, high-mass ions with large translational kinetic energies can be trapped efficiently and detected. Using this approach, low-resolution spectra of carbonic anhydrase (MW= 29 000), egg albumin (MW