Biased codon usage near intron-exon junctions: selection on splicing enhancers, splice-site recognition or something else?
Biased codon usage near intron-exon junctions: selection on splicing enhancers, splice-site recognition or something else?
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DOI：
10.1016/j.tig.2005.03.001
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发表时间：
2005-05-01
影响因子：
11.4
通讯作者：
Hurst, LD
中科院分区：
文献类型：
--
作者：
Chamary, JV;Hurst, LD
两个研究小组最近认为，在人类基因中，内含子-外显子连接附近的同义位点会经历正确剪接的选择。然而，两项研究都没有控制外显子末端潜在核苷酸偏差的可能性。在本文中，我们证明存在广义的 A 和 T 富集，这可能独立于剪接调控。在控制了这种偏差后，与剪接增强子相关的同义密码子之间的选择证据仍然存在，而对隐藏剪接位点避免的支持则减少了。
Two groups recently argued that, in human genes, synonymous sites near intron-exon junctions undergo selection for correct splicing. However, neither study controlled for the possibility of an underlying nucleotide bias at the ends of exons. In this article, we show that generalized A and T enrichment exists, which could be independent of splicing regulation. Evidence for selection between synonymous codons that are associated with splicing enhancers remains after controlling for this bias, whereas support for cryptic splice-site avoidance is diminished.