Contamination, distribution and pathogenicity of Toxocara canis and T. cati eggs from sandpits in Tokyo, Japan.
Contamination, distribution and pathogenicity of Toxocara canis and T. cati eggs from sandpits in Tokyo, Japan.
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日本东京沙坑中犬弓蛔虫和猫弓蛔虫卵的污染、分布和致病性。
DOI：
10.1017/s0022149x12000314
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Ohta N.
中科院分区：
文献类型：
--
作者：
Macuhova K;Akao N;Fujinami Y;Kumagai T;Ohta N.
本文报道了犬弓首线虫和弓首线虫的污染、分布和致病性。描述了日本东京大都会地区沙坑中的猫卵。共检测34个沙坑，其中14个沙坑被T.通过漂浮法和聚合酶链反应（PCR）分析评估猫卵。对两个自然污染的沙坑进行了调查，以确定卵的垂直和水平分布，并观察到砂深度和卵数量之间的反比关系。为了检测虫卵的致病性，用300个从沙坑中回收的虫卵接种3只ICR小鼠。感染3周后，小鼠外周血中出现嗜酸性粒细胞增多，血清中产生IgG抗体。大多数迁移的幼虫是从尸体中发现的，尽管在两只受感染的老鼠的大脑中发现了三只。经鉴定，这3头幼虫为T.犬T. cati和T.在沙坑中可以发现犬卵，而且从沙坑中回收的卵具有侵入寄生宿主的能力。
The contamination, distribution and pathogenicity of Toxocara canis and T. cati eggs in sandpits in the Tokyo metropolitan area, Japan, are described. A total of 34 sandpits were examined, 14 of which were contaminated with T. cati eggs, as assessed by the floatation method and polymerase chain reaction (PCR) analysis. Two naturally contaminated sandpits were investigated to determine the vertical and horizontal distribution of eggs, and an inverse relationship between the sand depth and number of eggs was observed. To examine the pathogenicity of the eggs, three ICR mice were inoculated with 300 eggs, which were recovered from sandpits. The mice exhibited eosinophilia in the peripheral blood and IgG antibody production in the sera after 3 weeks of infection. Most migrating larvae were recovered from carcasses, although three were found in the brains of two infected mice. These three larvae were determined to be T. canis by PCR, revealing that not only T. cati, but also T. canis eggs could be found in sandpits and, further, that eggs recovered from sandpits have the ability to invade a paratenic host.