Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria
Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria
复制标题
DOI：
10.1080/08927014.2014.996143
复制
发表时间：
2015-01-01
期刊：
影响因子：
2.7
通讯作者：
Williams, David Wynne
中科院分区：
文献类型：
--
作者：
Cavalcanti, Yuri Wanderley;Morse, Daniel James;Williams, David Wynne
本研究探讨了细菌对念珠菌生物被膜毒力和致病性的影响。成熟的生物膜(纯白色念珠菌、纯细菌、有细菌的白色念珠菌)在丙烯酸上产生，并直接进行分析，或用于感染重组的人类口腔上皮(RHOE)。分析包括念珠菌菌丝计数和念珠菌毒力基因表达的评估。同时检测乳酸脱氢酶(LDH)活性和RHOE生物膜感染后念珠菌的组织侵袭情况。菌丝念珠菌在含有细菌的丙烯酸酯生物膜中更为常见(p<；0.05)，分泌型天冬氨酸蛋白酶(SAP4/SAP6)和菌丝壁蛋白(HWP1)基因表达上调(p<；0.05)。念珠菌黏附素基因(ALS3/EPA1)、SAP6和HWP1在RHOE混合生物被膜感染中表达上调。多菌种感染表现出更高的菌丝比例(p<；0.05)、IL-18上调、更高的LDH活性和组织侵袭。由于丙烯酸酯生物膜中细菌的存在促进了念珠菌的毒力，因此在管理义齿生物膜相关假丝酵母菌时应考虑细菌成分。
This study examined the influence of bacteria on the virulence and pathogenicity of candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated on acrylic and either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration and assessment of Candida virulence gene expression. Lactate dehydrogenase (LDH) activity and Candida tissue invasion following biofilm infection of the RHOE were also measured. Candida hyphae were more prevalent (p < 0.05) in acrylic biofilms also containing bacteria, with genes encoding secreted aspartyl-proteinases (SAP4/SAP6) and hyphal-wall protein (HWP1) up-regulated (p < 0.05). Candida adhesin genes (ALS3/EPA1), SAP6 and HWP1 were up-regulated in mixed-species biofilm infections of RHOE. Multi-species infections exhibited higher hyphal proportions (p < 0.05), up-regulation of IL-18, higher LDH activity and tissue invasion. As the presence of bacteria in acrylic biofilms promoted Candida virulence, consideration should be given to the bacterial component when managing denture biofilm associated candidoses.