The Rhizosphere Responds: Rich Fen Peat and Root Microbial Ecology after Long-Term Water Table Manipulation
The Rhizosphere Responds: Rich Fen Peat and Root Microbial Ecology after Long-Term Water Table Manipulation
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DOI：
10.1128/aem.00241-21
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发表时间：
2021-04
影响因子：
4.4
通讯作者：
D. Rupp;L. J. Lamit;S. Techtmann;E. Kane;E. Lilleskov;M. Turetsky
中科院分区：
文献类型：
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作者：
D. Rupp;L. J. Lamit;S. Techtmann;E. Kane;E. Lilleskov;M. Turetsky
这项研究表明，北方沼泽的根部和生根区支持可能具有产甲烷、铁循环和真菌内生关联的生物体，并直接或间接影响这些生态系统中的碳循环。这些类群对地下水位的变化做出反应，并与根系相关，特别是禾本科植物，可能会获得更大的生物地球化学影响，因为预计较高的降水率可能会导致北方沼泽中莎草和草的丰度增加。摘要 气候变化导致的水文变化将影响北方泥炭地储存的碳 (C) 循环。这些系统中的碳循环是由密切相关的微生物和植物进行的。这项研究调查了实验控制的地下水位（降低和升高）和植物功能组对北方富含沼泽的泥炭和根微生物组的影响。所有样本均针对细菌、古菌（16S DNA 基因；V4）和真菌（内转录间隔区 2 [ITS2]）DNA 进行测序和处理。深度对所有地下水位处理中的微生物和真菌群落都有很大影响。细菌和古菌群落对地下水位处理最敏感，特别是在 10 至 20 厘米深度；该区域与根际或生根区重合。铁循环者，特别是地杆菌科的成员，在莎草、木贼和草的根部富集。真菌群落很大程度上受植物功能群的影响，尤其是五叶草。莎草和草根中富含真菌内生菌（特别是 Acephala spp.），这可能对有机物分解和循环具有未被充分认识的影响。真菌木质纤维素降解剂在降低地下水位的处理中得到富集。我们的结果表明了两个主要的产甲烷菌群落，一个以古菌科甲烷杆菌科为主的生根区群落和一个以甲烷微生物科为主的深层泥炭群落。重要性 这项研究表明，北方沼泽中的根和生根区支持可能具有产甲烷、铁循环和真菌内生关联的生物体，并直接或间接影响这些生态系统中的碳循环。这些类群对地下水位的变化做出反应，并与根系相关，特别是禾本科植物，可能会获得更大的生物地球化学影响，因为预计较高的降水率可能会导致北方沼泽中莎草和草的丰度增加。
This study demonstrated that roots and the rooting zone in boreal fens support organisms likely capable of methanogenesis, iron cycling, and fungal endophytic association and are directly or indirectly affecting carbon cycling in these ecosystems. These taxa, which react to changes in the water table and associate with roots and, particularly, graminoids, may gain greater biogeochemical influence, as projected higher precipitation rates could lead to an increased abundance of sedges and grasses in boreal fens. ABSTRACT Hydrologic shifts due to climate change will affect the cycling of carbon (C) stored in boreal peatlands. Carbon cycling in these systems is carried out by microorganisms and plants in close association. This study investigated the effects of experimentally manipulated water tables (lowered and raised) and plant functional groups on the peat and root microbiomes in a boreal rich fen. All samples were sequenced and processed for bacterial, archaeal (16S DNA genes; V4), and fungal (internal transcribed spacer 2 [ITS2]) DNA. Depth had a strong effect on microbial and fungal communities across all water table treatments. Bacterial and archaeal communities were most sensitive to the water table treatments, particularly at the 10- to 20-cm depth; this area coincides with the rhizosphere or rooting zone. Iron cyclers, particularly members of the family Geobacteraceae, were enriched around the roots of sedges, horsetails, and grasses. The fungal community was affected largely by plant functional group, especially cinquefoils. Fungal endophytes (particularly Acephala spp.) were enriched in sedge and grass roots, which may have underappreciated implications for organic matter breakdown and cycling. Fungal lignocellulose degraders were enriched in the lowered water table treatment. Our results were indicative of two main methanogen communities, a rooting zone community dominated by the archaeal family Methanobacteriaceae and a deep peat community dominated by the family Methanomicrobiaceae. IMPORTANCE This study demonstrated that roots and the rooting zone in boreal fens support organisms likely capable of methanogenesis, iron cycling, and fungal endophytic association and are directly or indirectly affecting carbon cycling in these ecosystems. These taxa, which react to changes in the water table and associate with roots and, particularly, graminoids, may gain greater biogeochemical influence, as projected higher precipitation rates could lead to an increased abundance of sedges and grasses in boreal fens.