Cycloheximide resistance in yeast: the gene and its protein.
Cycloheximide resistance in yeast: the gene and its protein.
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DOI：
10.1093/nar/11.10.3123
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发表时间：
1983-05
影响因子：
14.9
通讯作者：
N. F. Kaufer;H. M. Fried;W. F. Schwindinger;M. Jasin;Jonathan;R. Warner
中科院分区：
文献类型：
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作者：
N. F. Kaufer;H. M. Fried;W. F. Schwindinger;M. Jasin;Jonathan;R. Warner
酵母基因CYH2的突变可导致对真核蛋白合成抑制剂环己亚胺的抗性。CYH2的基因产物是核糖体蛋白L29，是60S核糖体亚基的一个组成部分。我们克隆了CYH2的野生型和抗性等位基因，并确定了它们的核苷酸序列。根据S1核酸酶分析，CYH2的转录似乎在多个位点开始和终止。该基因被转录成约1082个核苷酸的RNA分子，包含510个核苷酸的中间序列。内含子的剪接连接位于基因5'端附近的密码子内。在证实Stocklein等人(1)的肽分析中，我们发现对环己亚胺的抗性是由于L29位置37的谷氨酸取代了谷氨酰胺的翻转突变。
Mutations in the yeast gene CYH2 can lead to resistance to cycloheximide, an inhibitor of eukaryotic protein synthesis. The gene product of CYH2 is ribosomal protein L29, a component of the 60S ribosomal subunit. We have cloned the wild-type and resistance alleles of CYH2 and determined their nucleotide sequence. Transcription of CYH2 appears to initiate and terminate at multiple sites, as judged by S1 nuclease analysis. The gene is transcribed into an RNA molecule of about 1082 nucleotides, containing an intervening sequence of 510 nucleotides. The splice junction of the intron resides within a codon near the 5' end of the gene. In confirmation of peptide analysis by Stocklein et al. (1) we find that resistance to cycloheximide is due to a transversion mutation resulting in the replacement of a glutamine by glutamic acid in position 37 of L29.