Hypothermal effects on survival, energy homeostasis and expression of energy-related genes of swimming crabs Portunus trituberculatus during air exposure
Hypothermal effects on survival, energy homeostasis and expression of energy-related genes of swimming crabs Portunus trituberculatus during air exposure
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DOI：
10.1016/j.jtherbio.2016.06.007
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发表时间：
2016-08-01
影响因子：
2.7
通讯作者：
Dong, Shuanglin
中科院分区：
文献类型：
--
作者：
Lu, Yunliang;Zhang, Dan;Dong, Shuanglin
以往，在低温条件下的干或半干方法被证明是三疣梭子蟹活体运输的一种替代方法。然而，我们想知道这种方法是否能在温度保持尽可能低的情况下提高螃蟹的存活率。在这项研究中，我们假设有一个温度阈值，低于该温度阈值，干燥或半干燥的方法（空气暴露）可能会导致螃蟹生理中断，从而加剧其死亡率。为了验证上述假设，螃蟹（23摄氏度）暴露在4至16摄氏度的空气中。结果表明，温度低于12摄氏度，螃蟹的存活率和活力都很差。并通过对螃蟹能量代谢的测定，探讨其可能的原因.结果表明，低于12 ℃的空气暴露显著降低了鳃中腺嘌呤核苷酸和腺苷酸的总能荷。这增加了螃蟹重新平衡能量代谢的需要，这通过AMPK α和HIP-1 α的上调来表示。在12 ℃以下，Na+/K+-ATP酶、V型ATP酶和HSP 90的表达量显著增加，而血淋巴中血蓝蛋白、尿酸盐和乳酸盐的含量以及鳃中细胞色素c氧化酶和NADH-泛醌还原酶的表达量均无显著差异。这些结果表明，低于12 ℃的干或半干环境对三疣梭子蟹的生长具有不利影响，能量平衡失调可能是导致三疣梭子蟹死亡和活力下降的原因之一，而能量平衡失调与三疣梭子蟹需能生理途径的改变密切相关。(C)2016爱思唯尔有限公司版权所有
Previously, dry or semi-dry approach under the hypothermal condition is proved to be an alternative method in transport of live swimming crabs Portunus trituberculatus. However, we wondered whether this method can improve crab survival when temperature is kept as cool as possible. In this study, we hypothesized that there is a thermal threshold below which dry or semi-dry approach (air exposure) could cause crab physiological disruption and therefore aggravate their mortality. To test the above hypothesis, crabs (23 degrees C) were exposed to air at temperatures ranging from 4 to 16 degrees C. Results showed that crabs had a worse survival and vigor at temperatures below 12 degrees C. Then we tested crab energy metabolism to explore the possible reason. It was shown that total adenine nucleotide and adenylate energy charge in gills were remarkably reduced by air exposure of below 12 degrees C. This increased the need for crabs to re-balance energy metabolism, which was indicated by the upregulation of AMPK alpha and HIP-1 alpha. Meanwhile, there was a significant increase of the expression of Na+/K+-ATPase, V-type ATPase and HSP90 at temperatures below 12 degrees C, while all treatments shared a similar level of hemocyanin, urate and lactate in hemolymph and expression of cytochrome c oxidase and NADH-ubiquinone reductase in gills. These results implied that dry or semi-dry approach below 12 degrees C could exert detrimental effects on P. trituberculatus, and perturbation of energy homeostasis, which is more related with changes of energy demanding physiological pathways, is a possible reason of crab death and poor vigor. (C) 2016 Elsevier Ltd. All rights reserved.