Role of interleukin-12 in the development of acute graft-versus-host disease in bone marrow transplant patients
Role of interleukin-12 in the development of acute graft-versus-host disease in bone marrow transplant patients
复制标题
白细胞介素12在骨髓移植患者急性移植物抗宿主病发生中的作用
DOI：
--
复制
发表时间：
1999
影响因子：
4.8
通讯作者：
T. Nishimura
中科院分区：
文献类型：
--
作者：
M. Yabe;H. Yabe;K. Hattori;T. Shimizu;M. Matsumoto;T. Morimoto;Y. Yasuda;H. Inoue;S. Kato;T. Nishimura
白细胞介素-12（IL-12）是一种重要的细胞因子，调节细胞介导的免疫，并可能有助于移植物抗宿主病（GVHD）的发展。本研究观察了异基因造血干细胞移植受者外周血淋巴细胞（PBL）经IL-12+抗CD 3单克隆抗体（mAb）刺激后血清IL-12浓度和IFN-γ产生的变化。我们还研究了异基因干细胞受体和接受供体白细胞输注（DLT）治疗或预防白血病复发和EB病毒（EBV）淋巴组织增生性疾病（LPD）的患者在脂多糖（LPS）刺激后外周巨噬细胞（Mφ）产生IL-12的情况。急性GVHD患者的PBL在IL-12加抗CD 3 mAb刺激后产生高水平的IFN-γ，而非急性GVHD患者的PBL产生低水平的IFN-γ。然而，两组的血清IL-12浓度均较低。与未发生急性GVHD的患者相比，发生急性GVHD的患者外周血Mφ IL-12产生增加。5例接受DLT治疗或预防白血病复发的患者发生急性GVHD。在急性GVHD发生后，IL-12+抗CD 3单抗刺激PBL产生IFN-γ，而LPS刺激Mφ产生IL-12。然而，血清IL-12浓度仍然很低。3例接受DLT治疗EBV-LPD的患者未发生急性GVHD，IFN-γ和IL-12的产生也未增加。这些结果表明，IL-12可能在异基因干细胞移植或DLT后急性GVHD的发展中起重要作用，并且Mφ产生的IL-12增加与各种刺激有关，包括LPS。
Interleukin-12 (IL-12) is a crucial cytokine regulating cell-mediated immunity, and may contribute to the development of graft-versus-host disease (GVHD). We investigated serum IL-12 concentrations, interferon gamma (IFN-γ) production by peripheral blood lymphocytes (PBL) from allogeneic stem cell recipients after IL-12 plus anti-CD3 monoclonal antibody (mAb) stimulation. We also investigated IL-12 production by peripheral macrophages (Mφ) after lipopolysaccharide (LPS) stimulation from allogeneic stem cell recipients and patients receiving donor leukocyte transfusions (DLT) for treatment or prophylaxis of leukemia relapse and Epstein–Barr virus (EBV) lymphoproliferative disease (LPD). PBL from acute GVHD patients produced high IFN-γ levels after IL-12 plus anti-CD3 mAb stimulation, whereas PBL from patients without acute GVHD produced low levels of IFN-γ. However, serum IL-12 concentrations were low in both groups. Peripheral Mφ IL-12 production increased in patients who developed acute GVHD compared to patients without acute GVHD. Five patients receiving DLT for treatment or prophylaxis of leukemia relapse developed acute GVHD. IFN-γ production by PBL stimulated by IL-12 plus anti-CD3 mAb increased, while IL-12 production by peripheral Mφ stimulated by LPS was very high after the development of acute GVHD. However, serum IL-12 concentration remained low. Three patients receiving DLT for EBV-LPD did not develop acute GVHD with no increase of IFN-γ and IL-12 production. These results indicate that IL-12 may play an important role in the development of acute GVHD after allogeneic stem cell grafting or DLT, and increased IL-12 production by Mφ occurs with various stimuli, including LPS.
登录
查看更多内容
影响因子：
4.4
作者：
B. Perussia;S. Chan;Annalisa D'Andrea;K. Tsuji;D. Santoli;M. Pospíšil;D. Young;S. Wolf;Giorgio Trinchieri
通讯作者：
B. Perussia;S. Chan;Annalisa D'Andrea;K. Tsuji;D. Santoli;M. Pospíšil;D. Young;S. Wolf;Giorgio Trinchieri
用抗 CD5 单克隆抗体选择性清除骨髓 T 淋巴细胞：有效预防血液系统恶性肿瘤患者的移植物抗宿主病。
影响因子：
20.3
作者：
Antin,JH;Bierer,BE;Smith,BR;Ferrara,J;Guinan,EC;Sieff,C;Golan,DE;Macklis,RM;Tarbell,NJ;Lynch,E
通讯作者：
Lynch,E
影响因子：
20.3
作者：
M. Horowitz;R. Gale;P. Sondel;J. Goldman;J. Kersey;H. Kolb;A. Rimm;O. Ringdén;C. Rozman
通讯作者：
M. Horowitz;R. Gale;P. Sondel;J. Goldman;J. Kersey;H. Kolb;A. Rimm;O. Ringdén;C. Rozman
IL-12 对实验性病毒感染的反应和易感性的影响。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Orange,JS;Wolf,SF;Biron,CA
通讯作者：
Biron,CA
影响因子：
20.3
作者：
A. M. Marmont;M. Horowitz;R. Gale;K. Sobocinski;Robert;-C.;Ash;Dirk W. van Bekkum;Richard;-E.;Champlin;K. A. Dicke;J. Goldman;Robert A. Good;R. Herzig;Richard Hong;Tohru Masaoka;A. Rimm;Olle Ringdh;Bruno Wilhelm Speck;Roy;Weiner;M. Bortin
通讯作者：
A. M. Marmont;M. Horowitz;R. Gale;K. Sobocinski;Robert;-C.;Ash;Dirk W. van Bekkum;Richard;-E.;Champlin;K. A. Dicke;J. Goldman;Robert A. Good;R. Herzig;Richard Hong;Tohru Masaoka;A. Rimm;Olle Ringdh;Bruno Wilhelm Speck;Roy;Weiner;M. Bortin