Role of recently migrated monocytes in cigarette smoke-induced lung inflammation in different strain of mice.
Role of recently migrated monocytes in cigarette smoke-induced lung inflammation in different strain of mice.
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DOI：
10.1371/journal.pone.0072975
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peces-Barba G
中科院分区：
文献类型：
--
作者：
Pérez-Rial S;del Puerto-Nevado L;Terrón-Expósito R;Girón-Martínez Á;González-Mangado N;Peces-Barba G
本研究探讨了从血液循环中募集并在支气管肺泡灌洗（BAL）液中回收的促炎单核细胞在两种具有不同肺气肿易感性的小鼠（易感-C57 BL/6 J和非易感-129 S2/SvHsd）急性香烟烟雾（CS）诱导的肺部炎症模型中介导肺损伤的作用。在一天内连续3支研究香烟暴露于全身CS诱导小鼠肺部急性炎症。BAL液分析显示，与非易感小鼠相比，在CS暴露后72 h，易感小鼠中最近迁移的单核细胞流入更多。与C57 BL/6 J小鼠肺组织MMP-12和TNF-α蛋白水平的增加以及电泳迁移率改变分析测定的NF-κB向核转位的增加相关。为了确定这些促炎性单核细胞在介导CS诱导的气道炎症中的功能作用，通过在CS暴露前2天和4天给予气管内和静脉内脂质体包封的CL 2 MDP预处理暂时去除肺泡巨噬细胞和血液单核细胞，并研究其再增殖。单核细胞/巨噬细胞在最后一次脂质体应用后48 h最大限度地消耗，随后在CS暴露后72 h，最近迁移的单核细胞再次出现在易感小鼠的BAL液中。最近迁移的单核细胞流入肺与肺组织中MMP-12蛋白水平的增加相关，表明促炎单核细胞的增加与主要组织损伤相关。因此，我们的数据证实，从血液中招募最近迁移的促炎症单核细胞是导致MMP-12增加的原因，并且在急性肺部炎症诱导的肺部疾病的发病机制中发挥重要作用。这些结果可能有助于理解这些品系小鼠对CS的不同易感性。
This study investigates the role of proinflammatory monocytes recruited from blood circulation and recovered in bronchoalveolar lavage (BAL) fluid in mediating the lung damage in a model of acute cigarette smoke (CS)-induced lung inflammation in two strains of mice with different susceptibility to develop emphysema (susceptible -C57BL/6J and non susceptible -129S2/SvHsd). Exposure to whole-body CS for 3 consecutive research cigarettes in one single day induced acute inflammation in the lung of mice. Analysis of BAL fluid showed more influx of recently migrated monocytes at 72 h after CS-exposition in susceptible compared to non susceptible mice. It correlated with an increase in MMP-12 and TNF-α protein levels in the lung tissue, and with an increment of NF-κB translocation to the nucleus measured by electrophoretic mobility shift assay in C57BL/6J mice. To determine the functional role of these proinflammatory monocytes in mediating CS-induced airway inflammation, alveolar macrophages and blood monocytes were transiently removed by pretreatment with intratracheal and intravenous liposome-encapsulated CL2MDP, given 2 and 4 days prior to CS exposure and their repopulation was studied. Monocytes/macrophages were maximally depleted 48 h after last liposome application and subsequently recently migrated monocytes reappeared in BAL fluid of susceptible mice at 72 h after CS exposure. Recently migrated monocytes influx to the lung correlated with an increase in the MMP-12 protein level in the lung tissue, indicating that the increase in proinflammatory monocytes is associated with a major tissue damaging. Therefore our data confirm that the recruitment of proinflammatory recently migrated monocytes from the blood are responsible for the increase in MMP-12 and has an important role in the pathogenesis of lung disease induced by acute lung inflammation. These results could contribute to understanding the different susceptibility to CS of these strains of mice.
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影响因子：
15.3
作者：
Muller, W A
通讯作者：
Muller, W A
DOI：
10.1124/jpet.107.134007
发表时间：
2008-05-01
影响因子：
3.5
作者：
Sato, Atsuyasu;Hoshino, Yuma;Yodoi, Junji
通讯作者：
Yodoi, Junji
影响因子：
10
作者：
Demedts, IK;Morel-Montero, A;Brusselle, GG
通讯作者：
Brusselle, GG
影响因子：
4.8
作者：
Moodie, FM;Marwick, JA;Rahman, I
通讯作者：
Rahman, I
DOI：
10.1165/rcmb.2007-0224rc
发表时间：
2008-04-01
影响因子：
6.4
作者：
Murphy, Jaime;Summer, Ross;Fine, Alan
通讯作者：
Fine, Alan