Gastric bypass model in the obese rat to study metabolic mechanisms of weight loss
Gastric bypass model in the obese rat to study metabolic mechanisms of weight loss
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DOI：
10.1006/jsre.2002.6508
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发表时间：
2002-09-01
影响因子：
2.2
通讯作者：
Inui, A
中科院分区：
文献类型：
--
作者：
Xu, Y;Ohinata, K;Inui, A
本实验采用Zucker大鼠作为肥胖模型，分阶段建立了用于病态肥胖患者的Roux-en-Y（GB）胃旁路术大鼠模型。在最终模型中，使用两条缝钉线（Ethicon）创建了20%胃底袋以限制胃储液囊。一个4- 5-mm端侧胃空肠吻合术和一个6- 8-mm空肠空肠吻合术，Roux-en-Y！肢体，放置在Treitz韧带下16 cm处，手工缝合，以创建营养消化和吸收的有限区域。对照组行假手术。在两个实验中，将大鼠分为：（i）假手术自由进食（对照）;（ii）GB;和（iii）假手术成对进食（PF）。在实验1中，研究了24只Zuckers（对照组n = 8; GB n = 8; PF n = 8），以评估该模型对体重减轻的有效性。在实验2中，研究了24只Zuckers（8只/组），以确认手术对体重减轻和代谢参数的影响。从术后24小时开始给予加强4天，然后给予磨碎的食物。测量每日摄食量（FI）、餐量（MZ）、餐数（MN）和体重（BW）。在实验1的第20天和实验2的第10天处死大鼠。测定血清代谢物和体脂重量。使用Student t检验评价数据。对照组体重稳步增加（5.2-6.1 g/天），达到500 g左右。GB组：FI、MZ、MN、BW、血糖、游离脂肪酸、胰岛素、体脂均降低（P < 0.05）。PF：BW、胰岛素、甘油三酯和体脂降低。建立了一种可靠的、可重复的Roux-en-Y肥胖模型胃旁路术。这允许研究由手术引起的体重减轻的生物化学和最终分子机制。(C)2002年爱思唯尔科学（美国）。
A rat model replicating gastric bypass with Roux-en-Y (GB) as used in morbidly obese patients, evolved in our laboratory in stages, using the Zucker rat as an obese model (GB) is presented. In the final model, a 20% gastric fundic pouch to limit the gastric reservoir was created using two staple lines (Ethicon). A 4- to 5-mm end-to-side gastrojejunostomy and a 6- to 8-mm jejunojejunostomy, at 10 cm length of the Roux-en-Y! limb, placed 16 cm below the ligament of Treitz, was hand sewn to create a limited area of nutrient digestion and absorption. Controls underwent sham operation. Rats were divided into: (i) sham-op ad lib-fed (Control); (ii) GB; and (iii) sham-op pair fed (PF) in two experiments. In Experiment 1, 24 Zuckers (control n = 8; GB n = 8; PF n = 8) were studied to assess the effectiveness of the model for weight loss. In Experiment 2, 24 Zuckers (8/group) were studied to confirm the effects of the operation on weight loss and on metabolic parameters. Boost was given for 4 days starting 24 h postop and then ground chow was given. Daily food intake (FI), meal size (MZ), meal number (MN), and body weight (BW) were measured. Rats were sacrificed on Day 20 in Experiment 1 and on Day 10 in Experiment 2. Serum metabolites and body fat weight were measured. Data were evaluated using Student's t test. Controls steadily gained BW (5.2-6.1 g/day), reaching similar to500 g. In GB: FI, MZ, MN, BW, glucose, free fatty acids, insulin, and body fat decreased (P < 0.05). In PF: BW, insulin, triglycerides, and body fat decreased. A dependable, reproducible gastric bypass with Roux-en-Y obesity model was developed. This permits the study of biochemical and eventually molecular mechanism(s) of weight loss resulting from the operation. (C) 2002 Elsevier Science (USA).