Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging.
Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging.
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DOI：
10.1364/boe.3.001274
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发表时间：
2012-06-01
影响因子：
3.4
通讯作者：
Girkin JM
中科院分区：
文献类型：
--
作者：
Saunter CD;Semprini S;Buckley C;Mullins J;Girkin JM
在本文中，我们报告了一种无扫描仪探针的设计，测试和使用，专门用于体内深部组织的微创成像，具有外显荧光模式。探针通过710 μm外径的探针成像直径500 μm的视场，最大组织穿透深度为15 mm，专为eGFP成像配置。用2.5 μm的横向分辨率对大鼠和斑马鱼的脑垂体进行了成像。
In this paper we report the design, testing and use of a scannerless probe specifically for minimally invasive imaging of deep tissue in vivo with an epi-fluorescence modality. The probe images a 500 μm diameter field of view through a 710 μm outer diameter probe with a maximum tissue penetration depth of 15 mm specifically configured for eGFP imaging. Example results are given from imaging the pituitary gland of rats and zebrafish hearts with lateral resolution of 2.5 μm.