Variation in chalcononaringenin 2'-O-glucoside content in the petals of carnations (Dianthus caryophyllus) bearing yellow flowers
Variation in chalcononaringenin 2'-O-glucoside content in the petals of carnations (Dianthus caryophyllus) bearing yellow flowers
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DOI：
10.1016/s0304-4238(03)00093-1
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发表时间：
2004-02
影响因子：
4.3
通讯作者：
H. Yoshida;Y. Itoh;Y. Ozeki;T. Iwashina;M. Yamaguchi
中科院分区：
文献类型：
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作者：
H. Yoshida;Y. Itoh;Y. Ozeki;T. Iwashina;M. Yamaguchi
查耳酮肾上腺素2′-O-葡萄糖苷（Chalcononaringenin 2′-O-glucoside，Ch 2 ′G）是开黄花康乃馨花瓣中的主要色素分子。31个香石竹基因型中该色素的含量在5.5 ~ 100.0%之间（以7154-03品系为100%）。苯丙氨酸解氨酶（PAL）和查尔酮合成酶（CHS）基因的转录都是活跃的黄色康乃馨花和一个氰控制品种的花瓣。黄色香石竹花瓣中查尔酮-黄烷酮异构酶（CHI）基因的转录产物在北方杂交分析中检测不到，但在RT-PCR中可以检测到。这可能产生微量的CHI蛋白，其从可疑量的mRNA翻译以催化查耳酮，导致Ch 2 ′G浓度的变化。其他可能的因素包括提供类黄酮生物合成途径的底物的量、流过CHI步骤产生黄酮醇衍生物的自发异构化和查耳酮2′-葡萄糖基转移酶（CHGT）活性。
Chalcononaringenin 2′-O-glucoside (Ch2′G) was found to be the major pigment molecule in the petals of carnations bearing yellow flowers. The concentration of this pigment varied from 5.5 to 100.0% (relative value with amounts in the line ‘7154-03’ assumed to be 100%) in 31 carnation genotypes investigated. The transcription of both phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) genes was active in the petals of both yellow carnation flowers and a cyanic control cultivar. The transcripts derived from the chalcone–flavanone isomerase (CHI) gene in the petals of yellow carnation flowers were below the level detectable by Northern blot analysis, but could be detected by RT-PCR. This is possible to produce subtle amounts of CHI protein translated from suspicious amounts of the mRNA to catalyze chalcone, resulting in the variation of the concentration of Ch2′G. Other probable factors caused to the variation include the amount of substrates supplying the flavonoid biosynthetic pathway, spontaneous isomerization flowing over the CHI step producing flavonol derivatives, and chalcone 2′-glucosyltransferase (CHGT) activity.