Multilayer micromolding of degradable polymer tissue engineering scaffolds
Multilayer micromolding of degradable polymer tissue engineering scaffolds
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DOI：
10.1016/j.msec.2007.04.021
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发表时间：
2008-04-01
期刊：
影响因子：
--
通讯作者：
Hansford, Derek J.
中科院分区：
文献类型：
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作者：
Gallego, Daniel;Ferrell, Nicholas;Hansford, Derek J.
精确的表面几何形态已被证明可以改善细胞增殖、粘附和功能。已经发现，细胞对其大小的一小部分的特征尺寸反应强烈。本文将软光刻技术应用于精确控制微尺度图案的聚二甲基硅氧烷微加工模具。采用多层微模塑法制备了三维聚己内酯（PCL）支架。研究了微成型PCL的加热和冲压工艺参数。这个过程可以控制支架内支撑结构的大小、形状和间距。具有独立特征的多层微成型允许层之间对齐。高孔隙率、丰富的互连和锋利的特征是支架的固有优势。将人骨肉瘤细胞植入三维支架中进行细胞生长试验。荧光显微镜和扫描电镜显示，细胞对3-13个支架反应良好，支架调节细胞形态和粘附。(c) 2007 Elsevier B.V.版权所有
Precise surface geometrical morphologies have been shown to improve cellular proliferation, adhesion, and functionality. It has been found that cells respond strongly to feature dimensions a fraction of their size. In this paper, soft lithography techniques were applied to microfabricate polydimethylsiloxane molds with precisely controlled micro-scale patterns. Three-dimensional polycaprolactone (PCL) scaffolds were fabricated using a multilayer micromolding (MMM) method. Proper heating and stamping parameters were developed for micromolding PCL. This process allowed control of the size, shape, and spacing of support structures within the scaffold. The micromolding of multiple layers with independent features allowed for alignment between layers. The high porosity, abundant interconnections, and sharp features were inherent advantages of the scaffolds. Human osteosarcoma cells were seeded in the 3-D scaffolds for cell growth testing. Fluorescent microscopy and scanning electron micrographs showed that cells responded well to the 3-13 scaffolds and the scaffolds regulated cell morphology and adhesion. (c) 2007 Elsevier B.V. All rights reserved.