Isolation of Human Neutrophils from Venous Blood
Isolation of Human Neutrophils from Venous Blood
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DOI：
10.1007/978-1-0716-0154-9_3
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Nauseef, William M.
中科院分区：
文献类型：
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作者：
Kremserova, Silvie;Nauseef, William M.
静脉血提供了大量未经刺激的粒细胞和单核细胞的现成来源。利用静脉血中循环的白细胞相对密度的差异，人们可以将白细胞从红细胞中分离出来，并分离出高纯度的单个白细胞群体，用于体外研究。对于选定的功能研究，如转录分析或细胞因子定量，在标准分离方案中添加免疫磁性阴性选择步骤可以获得高度纯化的人中性粒细胞。
Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies. For selected functional studies, such as transcriptional analysis or cytokine quantitation, addition of an immunomagnetic negative selection step to the standard isolation protocol can yield highly purified human neutrophils.