IMIDAZOLE-BUFFERED OSMIUM-TETROXIDE - AN EXCELLENT STAIN FOR VISUALIZATION OF LIPIDS IN TRANSMISSION ELECTRON-MICROSCOPY
IMIDAZOLE-BUFFERED OSMIUM-TETROXIDE - AN EXCELLENT STAIN FOR VISUALIZATION OF LIPIDS IN TRANSMISSION ELECTRON-MICROSCOPY
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DOI：
10.1007/bf01033631
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
FAHIMI, HD
中科院分区：
文献类型：
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作者：
ANGERMULLER, S;FAHIMI, HD
本文研究了咪唑缓冲四氧化锇在透射电子显微镜中作为脂类染色剂的有效性。用戊二醛灌注固定大鼠肝脏和其他组织，用锇-咪唑固定后，将脂滴的外观与固定后在未缓冲的四氧化锇水溶液或其他缓冲的锇溶液中进行比较。后固定2%锇咪唑，pH 7.5，为30分钟后，脂滴和脂蛋白颗粒的显着的电子不透明染色。脂滴出现良好的限制，没有扩散的证据。染色的强度要小得多，并且在水性或cacodylate缓冲的四氧化锇中固定后的材料中的脂滴周围存在一些扩散。滤纸上的斑点试验表明，不饱和脂肪酸，尤其是亚麻酸和亚油酸与锇咪唑的反应比与四氧化锇水溶液的反应更强烈。显然，锇-咪唑在透射电子显微镜中为脂质提供了极好的染色剂，并且最有可能的是它使具有不饱和脂肪酸的脂质染色。
The usefulness of imidazole-buffered osmium tetroxide as a stain for lipids in transmission electron microscopy was investigated. Rat liver and other tissues were fixed by perfusion with glutaraldehyde and post-fixed with osmium-imidazole and the appearance of lipid droplets was compared with that after post-fixation in unbuffered aqueous osmium tetroxide or an osmium solution buffered otherwise. Prominent electron-opaque staining of lipid droplets and of lipoprotein particles was noted after post-fixation with 2% osmium-imidazole, pH 7.5, for 30 min. The lipid droplets appeared well circumscribed with no evidence of diffusion. The intensity of staining was much less and there was some diffusion around lipid droplets in material post-fixed in aqueous or cacodylate-buffered osmium tetroxide. Spot tests on filter paper revealed that unsaturated fatty acids, especially linolenic and linoleic acids reacted more intensely with osmium-imidazole than with aqueous osmium tetroxide. Apparently, osmium-imidazole provides an excellent stain for lipids in transmission electron microscopy and that most probably it stains lipids with unsaturated fatty acids.