Interactive contributions of intracellular calcium and protein phosphatases to massed-trials learning deficits in Hermissenda.
Interactive contributions of intracellular calcium and protein phosphatases to massed-trials learning deficits in Hermissenda.
复制标题
细胞内钙和蛋白磷酸酶对 Hermissenda 大规模试验学习缺陷的交互作用。
DOI：
10.1037//0735-7044.113.1.103
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发表时间：
1999
影响因子：
1.9
通讯作者：
Matzel,LD
中科院分区：
文献类型：
--
作者：
Muzzio,IA;Ramirez,RR;Talk,AC;Matzel,LD
以Hermissenda为受试者，对大规模试验训练缺陷进行了检查。随着审判间隔（ITI）的延长（长达8分钟），光和旋转的相关配对会诱导对光反应的条件性足部收缩逐渐增强。相比之下，较短的ITI（30 s）没有产生学习的证据。在一个相应的体外条件反射实验，模拟训练的完整的动物，促进神经元兴奋性的动物的B光感受器在体内获得的结果。Fura-2对细胞内Ca 2+的显像显示，在短暂的ITI期间，Ca 2+水平仍保持较高水平.这种Ca 2+积累似乎诱导蛋白磷酸酶的激活，因为如果在磷酸酶抑制剂存在下进行训练，则用短ITI诱导B光感受器的正常易化。这些结果表明，细胞内Ca 2+和蛋白磷酸酶的相互作用有助于记忆形成的动力学，并提供证据，细胞内Ca 2+的积累，在整个训练试验可能会阻碍记忆的形成。（PsycINFO数据库记录（c）2016阿帕，保留所有权利）
Using Hermissenda as subjects, massed-trials training deficits were examined. Associative pairings of light and rotation induced a progressively greater conditioned foot contraction in response to light as the intertrial interval (ITI) was extended (up to 8 min). In contrast, a short ITI (30 s) produced no evidence of learning. In a corresponding in vitro conditioning experiment that mimicked training of the intact animal, facilitation of neuronal excitability in the animal's B photoreceptors paralleled the results obtained in vivo. Imaging of intracellular Ca 2+ using Fura-2 indicated that Ca 2+ levels remained elevated during short ITIs. This Ca 2+ accumulation appears to induce activation of protein phosphatases because normal facilitation of the B photoreceptors was induced with a short ITI if training occurred in the presence of a phosphatase inhibitor. These results suggest that intracellular Ca 2+ and protein phosphatases contribute interactively to the kinetics of memory formation and provide evidence that an accumulation of intracellular Ca 2+ across training trials may impede memory formation.(PsycINFO Database Record (c) 2016 APA, all rights reserved)