A pathogenetic study of the early connective tissue lesions of viral caprine arthritis-encephalitis.
A pathogenetic study of the early connective tissue lesions of viral caprine arthritis-encephalitis.
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病毒性山羊关节炎脑炎早期结缔组织病变的发病机制研究。
DOI：
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发表时间：
1980
影响因子：
6
通讯作者：
P. Klevjer
中科院分区：
文献类型：
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作者：
D. S. Adams;T. Crawford;P. Klevjer
实验旨在将形态学病变与山羊关节炎脑炎病毒（CAEV）的存在相关联。 21只剖腹产山羊羔感染10（6）至10（7）TCID50的病毒，依次处死，通过免疫荧光检查病毒抗原，通过分离和滴定检查病毒感染性，并通过光学显微镜检查形态变化。接种后 1 至 10 天 (DPI) 检测到荧光病毒抗原，并且仅在滑膜细胞中检测到。在 0.5 至 79 DPI 下从多个关节和大脑中重新分离出病毒。 1 DPI 时观察到滑液细胞计数增加，3 至 45 DPI 时滑膜形态发生变化。关节病变由轻度滑膜细胞增生和血管周围单核细胞浸润进展为重度滑膜细胞增生和单核细胞浸润伴绒毛肥厚。其他部位的病变较轻微，仅由血管周围单核细胞浸润组成。 11 只剖腹产对照山羊的病毒抗原、病毒和形态学病变均呈阴性。
Experiments were designed to correlate morphologic lesions with the presence of caprine arthritis-encephalitis virus (CAEV). Twenty-one cesarean-derived goat kids were infected with 10(6) to 10(7) TCID50 of virus, killed sequentially, and examined for viral antigens by immunofluorescence, viral infectivity by isolation and titration, and morphologic changes by light microscopy. Fluorescent viral antigens were detected from 1 to 10 days postinoculation (DPI) and only in synovial cells. Virus was reisolated from several joints and from brain 0.5 to 79 DPI. Increases in synovial fluid cell counts were noted by 1 DPI, and morphologic changes in synovial membranes were present from 3 to 45 DPI. Joint lesions progressed from mild synovial cell hyperplasia and perivascular mononuclear cell infiltration to severe synovial cell hyperplasia and mononuclear cell infiltration with villous hypertrophy. Lesions elsewhere were mild, consisting only of perivascular mononuclear cell infiltrates. Eleven cesarean-derived control goats were negative for viral antigens, virus, and morphologic lesions.