Bioconversion of wheat bran into high value-added products and modelling of fermentations
Bioconversion of wheat bran into high value-added products and modelling of fermentations
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DOI：
10.1016/j.indcrop.2019.111565
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发表时间：
2019-11-01
影响因子：
5.9
通讯作者：
Turhan, Irfan
中科院分区：
文献类型：
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作者：
Germec, Mustafa;Ozcan, Ali;Turhan, Irfan
本研究的目的是确定麦麸（WB）的粒径，优化预处理条件，并从酸预处理和未解毒的麦麸中生产乙醇（EtOH）、乳酸（LA）和菊粉酶（lase）。另一个目的是利用数学模型预测发酵的实验生产数据。结果表明，在固定温度（121℃）下，WB的最佳酸前处理条件为预处理时间1 min，稀酸比1.99% (v/v)，液固比11.97:1 （v/w）。在最佳条件下，还原糖、酚类物质、葡萄糖、木糖、阿拉伯糖、乙酸、5-羟甲基糠醛、2-糠醛的含量和催化效率（E， g糖/g抑制剂）分别为41.08 g/L （0.49 g糖/g生物量）、468.10 mg/L、17.66 g/L、6.93 g/L、3.78 g/L、0 g/L、247.84 mg/L、7.27 mg/L和39.28 g/g。以游离的stipitis Scheffersomyces （ATCC 58,784）和固定化干酪乳杆菌为原料，EtOH和LA的产率分别为30.44% （3.85 g/L）和66.68% （9.09 g/L）。而黑曲霉A42的激光酶活性(I)为116.85 U/mL，转化酶型活性(S)为44.88 U/mL， S/I比值为0.38。在数学建模方面，Huang模型比Baranyi模型对试验产量数据的预测更为成功。总的来说，WB可以作为微生物工艺生产增值产品的良好底物来源。
The objectives of this study were to determine the particle size of wheat bran (WB), to optimize the pretreatment conditions of WB and to produce ethanol (EtOH), lactic acid (LA), and inulinase (lase) from acid-pretreated and non-detoxified wheat bran. Another objective was to predict the experimental production data of the fermentations using mathematical models. It was found that the optimum acid-pretreatment conditions of WB were 1 min of pretreatment time, 1.99% (v/v) dilute-acid ratio, and 11.97:1 of liquid-to-solid ratio (v/w) at a fixed temperature (121 degrees C). Under optimal conditions, the levels of reducing sugar, phenolics, glucose, xylose, arabinose, acetic acid, 5-hydroxymethyl furfural, 2-furaldehyde and the catalytic efficiency (E, g sugar/g inhibitor) were 41.08 g/L (0.49 g sugar/g biomass), 468.10 mg/L, 17.66 g/L, 6.93 g/L, 3.78 g/L, 0 g/L, 247.84 mg/L, 7.27 mg/L, and 39.28 g/g, respectively. Maximum EtOH and LA yields were obtained as 30.44% (3.85 g/L, 59.57% of theoretical yield) and 66.68% (9.09 g/L) when the free Scheffersomyces stipitis (ATCC 58,784) and the immobilized Lactobacillus casei were used, respectively. Nevertheless, lase activity (I), invertase-type activity (S) and S/I ratio were 116.85 U/mL, 44.88 U/mL and 0.38 when Aspergillus niger A42 was employed, respectively. As concerning mathematical modeling, Huang model was more successful than Baranyi model in predicting the experimental production data. Overall, WB can be used as a good substrate source for production of value-added products by microbial processes.