Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231
Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231
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DOI：
10.1016/j.phymed.2009.12.008
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发表时间：
2010-07-01
期刊：
影响因子：
7.9
通讯作者：
Arunasree, K. M.
中科院分区：
文献类型：
--
作者：
Arunasree, K. M.
目的：虽然香芹酚的抗肿瘤作用已在早期得到证实，但涉及其作用的确切的潜在分子机制尚未确定，在本研究中，试图鉴定香芹酚诱导人转移性乳腺癌细胞MDA-MB 231中细胞死亡的机制。采用MTT法、Annexin V法、线粒体膜电位法、多酶活性测定法和流式细胞仪分析细胞周期，检测香芹酚诱导的细胞凋亡。结果：香芹酚诱导MDA-MB 231细胞凋亡呈剂量依赖性，IC 50为100 μ M，细胞线粒体膜电位降低，导致细胞色素c从线粒体释放，半胱天冬酶激活和PARP裂解。结论：香芹酚诱导MDA-MB 231细胞凋亡的IC 50为100 μ M。本研究中的数据清楚地证明了香芹酚对人转移性乳腺癌细胞MDA-MB 231的抗肿瘤作用，并且该化合物在治疗癌症方面可能具有潜在的治疗意义。(C)2009年Elsevier GmbH。All rights reserved.
Purpose: Although the anti-tumor effects of carvacrol have been demonstrated earlier, the exact underlying molecular mechanisms involved in its action have not been defined and in the present study an attempt has been made to identify the mechanism of carvacrol induced cell death in human metastatic breast cancer cells, MDA-MB 231.Methods: Apoptosis induced by carvacrol was determined based on different assays like MTT assay, Annexin V, mitochondrial membrane potential assay, multicaspase activation assay and cell cycle analysis by flow cytometer. Cleavage of PARP, cytochrome c release and modulation of Bax and Bcl2 ratio by Western blot analysis were also studied.Results: The study clearly showed induction of apoptosis by carvacrol in MDA-MB 231 cells dose dependently at an IC50 of 100 mu M with a decrease in the mitochondrial membrane potential of the cells resulting in release of cytochrome c from mitochondria, caspase activation and cleavage of PARP.Conclusion: The data in the present study clearly demonstrated anti-tumor effects of carvacrol on human metastatic breast cancer cells, MDA-MB 231, and that the compound could have a potential therapeutic significance in treating cancer. (C) 2009 Elsevier GmbH. All rights reserved.