Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice
Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice
复制标题
自噬对水稻花粉发育过程中基因表达及绒毡层程序性细胞死亡的影响
DOI：
10.3389/fpls.2020.00172
复制
发表时间：
2020-03-06
影响因子：
5.6
通讯作者：
Kuchitsu, Kazuyuki
中科院分区：
文献类型：
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作者：
Hanamata, Shigeru;Sawada, Jumpei;Kuchitsu, Kazuyuki
自噬是水稻绒毡层细胞程序性死亡（PCD）和花粉成熟所必需的。转录调控网络也被认为在绒毡层PCD的进展中发挥关键作用。然而，水稻花药发育中基因调控网络与自噬的关系尚不清楚。本研究利用高通量RNA测序技术，全面分析了自噬破坏对水稻花药发育中绒毡层PCD过程中基因表达谱的影响。数千个基因的表达，包括特定的转录因子和绒毡层降解所需的几种蛋白酶，波动同步在特定阶段在绒毡层PCD进展的野生型花药，而这种波动表现出显着的延迟自噬缺陷突变体Osatg 7 -1。基因本体富集分析结合自组织图聚类和途径分析表明，Osatg 7 -1突变体在花粉成熟过程中影响了多种细胞器相关基因以及糖/脂代谢相关基因的表达模式。这些结果表明，在水稻花粉发育过程中，自噬是基因表达和细胞器质量控制以及绒毡层PCD及时进展所必需的。
Autophagy has recently been shown to be required for tapetal programmed cell death (PCD) and pollen maturation in rice. A transcriptional regulatory network is also known to play a key role in the progression of tapetal PCD. However, the relationship between the gene regulatory network and autophagy in rice anther development is mostly unknown. Here, we comprehensively analyzed the effect of autophagy disruption on gene expression profile during the tapetal PCD in rice anther development using high-throughput RNA sequencing. Expression of thousands of genes, including specific transcription factors and several proteases required for tapetal degradation, fluctuated synchronously at specific stages during tapetal PCD progression in the wild-type anthers, while this fluctuation showed significant delay in the autophagy-deficient mutant Osatg7-1. Moreover, gene ontology enrichment analysis in combination with self-organizing map clustering as well as pathway analysis revealed that the expression patterns of a variety of organelle-related genes as well as genes involved in carbohydrate/lipid metabolism were affected in the Osatg7-1 mutant during pollen maturation. These results suggest that autophagy is required for proper regulation of gene expression and quality control of organelles and timely progression of tapetal PCD during rice pollen development.