Structural and quantitative analysis of astrocytes in the mouse hippocampus
Structural and quantitative analysis of astrocytes in the mouse hippocampus
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DOI：
10.1016/s0306-4522(02)00041-6
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Kosaka, T
中科院分区：
文献类型：
--
作者：
Ogata, K;Kosaka, T
利用光学显微镜、激光共聚焦扫描显微镜和高压电子显微镜观察小鼠海马星形胶质细胞的结构特征，并计算其数密度。高尔基体浸渍的星形胶质细胞的高压电子显微镜检查清楚地揭示了他们的罚款小叶状的过程中所占据的个别星形胶质细胞的群众。将两种不同的荧光示踪剂注射到两个相邻的星形胶质细胞中，发现每个星形胶质细胞仅在其外周部分占据了有限重叠的离散区域。在定量分析中使用的光学disector，确定为S100免疫反应阳性细胞的星形胶质细胞的数量密度仅略有不同，在其面积和层状分布。在陷窝-分子层和齿状门区数密度较高，而在主细胞层数密度略低于整个海马区的平均数（24.2 × 10 ~（-3）mm ~（-3））。在背腹侧差异中，齿状回腹侧水平的数密度显著大于海马，而海马本体的数密度差异不明显。高尔基体浸渍的星形胶质细胞的投影面积与其数密度大致成反比，腔隙分子层的面积略小于其他数密度高的层，表明星形胶质细胞分布较均匀，无明显的面积或层间差异，单个星形胶质细胞有自己的区域;给定星形胶质细胞的结构域的外围与相邻星形胶质细胞的突起错综复杂地相互交叉，而其内核部分不被它们穿透。(C)2002年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
We revealed the structural features of astrocytes by means of light microscopy, confocal laser scanning microscopy and high voltage electron microscopy, and estimated their numerical densities in the mouse hippocampus. The high voltage electron microscope examinations of Golgi-impregnated astrocytes clearly disclosed their fine leaflet-like processes in the masses occupied by individual astrocytcs. The intracellular injection of two different fluorescent tracers into two neighboring astrocytes revealed that each astrocyte occupied a discrete area with a limited overlap only at its peripheral portion. In a quantitative analysis using an optical disector, the numerical densities of astrocytes identified as S100-immunoreactive cells were only slightly different in their areal and laminar distributions. The numerical densities were higher in the stratum lacunosum-moleculare and dentate hilus, while they were slightly lower in the principal cell layers than the average (24.2 X 10(3) mm(-3)) in whole hippocampal regions. As for the dorsoventral difference, the numerical densities were significantly larger at the ventral level in the dentate gyrus, whereas such tendency was not apparent in the hippocampus proper. The projection area of the astrocytes estimated from Golgi-impregnated samples was roughly in inverse relation to the numerical densities; the areas in the stratum lacunosum-moleculare were somewhat smaller than the other layers, where the numerical densities were high.The present study indicates that astrocytes are distributed rather evenly without any prominent areal or laminar differences and that the individual astrocytes have their own domains; the periphery of the domain of a given astrocyte is interdigitated intricately with the processes of adjacent astrocytes whereas its inner core portion is not penetrated by them. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.