Two Highly Pathogenic Avian Influenza H5N1 Viruses of Clade 2.3.2.1 with Similar Genetic Background but with Different Pathogenicity in Mice and Ducks
Two Highly Pathogenic Avian Influenza H5N1 Viruses of Clade 2.3.2.1 with Similar Genetic Background but with Different Pathogenicity in Mice and Ducks
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DOI：
10.1111/j.1865-1682.2012.01325.x
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发表时间：
2013-04-01
影响因子：
4.3
通讯作者：
Liu, X.
中科院分区：
文献类型：
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作者：
Hu, J.;Zhao, K.;Liu, X.
禽流感病毒（AIV）在不同宿主中的毒力已经确定了许多遗传标记。然而，我们分离到两株H5 N1禽流感病毒，A/Chicken/Jiangsu/k 0402/2010（CK/10）和A/Goose/Jiangsu/k 0403/2010（GS/10），它们具有相似的遗传背景，但在两种病毒基因组中均未发现大多数明确的哺乳动物和禽类毒力分子标记。此外，这对病毒在不同宿主中的致病性尚不清楚。因此，我们评估了它们在鸡、小鼠、鸭和豚鼠中的致病性。感染CK/10和GS/10的鸡在24 h内死亡率均为100%。小鼠实验表明，CK/10为高致病性（MLD 50 =0.33 log 10 EID 50），GS/10为无毒性（MLD 50>6.32 log 10 EID 50）。CK/10对鸭的毒力（DLD 50 =3.83 log 10 EID 50）高于GS/10（DLD 50 =7.7 log 10 EID 50），这与病毒对小鼠的致病性有关。虽然CK/10和GS/10在小鼠中显示出不同的致病性，但它们对豚鼠都是致命的，其中CK/10在气道中复制到比GS/10更高的滴度。总之，这些发现表明，具有相似遗传背景的AIV可能在特定宿主中表现出不同的致病性，并且可能存在一些未知的毒力分子标记，需要进行鉴定。
A number of genetic markers for virulence of avian influenza viruses (AIVs) in different hosts have been identified. However, we isolated two H5N1 AIVs, A/Chicken/Jiangsu/k0402/2010(CK/10) and A/Goose/Jiangsu/k0403/2010(GS/10) with similar genetic background, but most well-defined molecular markers for virulence in mammals and avian species were not found in both viral genomes. In addition, pathogenicity of this pair of viruses in different hosts remains unclear. Therefore, we evaluated their pathogenicity in chickens, mice, ducks and guinea pigs. Infection of CK/10 and GS/10 in chickens caused 100% mortality within 24h. Mouse experiment showed that CK/10 was highly pathogenic (MLD50=0.33 log10 EID50), whereas GS/10 was avirulent (MLD50>6.32 log10 EID50). Interestingly, the virulence of CK/10 in ducks (DLD50=3.83 log10 EID50) was higher than that of GS/10 (DLD50=7.7 log10 EID50), which correlated with viral pathogenicity in mice. Although CK/10 and GS/10 showed distinct pathogenicity in mice, they both were lethal to guinea pigs, with CK/10 replicating to higher titres in airways than GS/10. Collectively, these findings suggest that AIVs with similar genetic backgrounds may exhibit distinct pathogenicity in specific hosts and that some unknown molecular markers for virulence may exist and need to be identified.