COMPARISON OF PROSTANOIDS AND THEIR PRECURSOR FATTY-ACIDS IN HUMAN HEPATOCELLULAR-CARCINOMA AND NONCANCEROUS REFERENCE TISSUES
COMPARISON OF PROSTANOIDS AND THEIR PRECURSOR FATTY-ACIDS IN HUMAN HEPATOCELLULAR-CARCINOMA AND NONCANCEROUS REFERENCE TISSUES
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DOI：
10.1006/jsre.1993.1010
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发表时间：
1993-01-01
影响因子：
2.2
通讯作者：
YURA, J
中科院分区：
文献类型：
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作者：
HANAI, T;HASHIMOTO, T;YURA, J
在人癌组织和参考肝组织中测定类二十烷酸的产生和前体脂肪酸的组成。对17例肝切除病例（12例肝细胞癌（HCC）和5例转移性肝癌）进行了评价。将癌组织和非癌参考组织分离，提取脂质，并通过气相色谱法测定脂肪酸甲酯。用放射免疫法测定前列腺素（6-ketoPGF 1 α和TXB 2）。在肝癌中，肝癌组织中α-亚麻酸（ω-3）（0.41 ± 0.38 × 100 μg/g）和二十二碳六烯酸（10.41 ± 4.96 × 100 μg/g）的水平明显低于参考组织。HCC组织中TXB 2（1.86 ± 2.77 pg/mg湿重）和6-ketoPGF 1 α水平均高于正常组织10倍。我们推测在HCC中，肝脏中较高的前列腺素水平部分与肿瘤代谢有关。
Eicosanoid production and the compositions of precursor fatty acids were determined in human cancerous and reference liver tissues. Seventeen hepatectomized cases (12 cases of hepatocellular carcinoma (HCC) and 5 cases of metastatic liver cancer) were evaluated. The cancerous tissues and the noncancerous reference tissues were separated, lipids were extracted, and the fatty acids were determined as methyl esters by gas chromatography. Prostanoids (6-keto PGF1αand TXB2) were measured by radioimmunoassay. In HCC, the levels of α-linolenic acid (ω-3) (0.41 ± 0.38 × 100 μg/g) and dochosahexaenoic acid (10.41 ± 4.96 × 100 μg/g) in liver cancer tissue were significantly less than those in the reference tissues. In HCC, the levels of TXB2reference (1.86 ± 2.77 pg/mg wet weight) and 6-keto PGF1αwere 10-fold higher than those in reference tissues. We speculate that in HCC higher prostanoid levels in the liver are related in part to tumor metabolism.