INDUCTION OF PROTO-ONCOGENE JUN AP-1 BY SERUM AND TPA
INDUCTION OF PROTO-ONCOGENE JUN AP-1 BY SERUM AND TPA
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DOI：
10.1038/334629a0
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发表时间：
1988-08-18
期刊：
影响因子：
64.8
通讯作者：
VERMA, IM
中科院分区：
文献类型：
--
作者：
LAMPH, WW;WAMSLEY, P;VERMA, IM
细胞对有丝分裂剂和分化剂的反应涉及几种细胞基因的转录诱导。这些所谓的“立即早期”或“能力”基因中最突出的是核癌基因fosandmycl-7。尽管这些早期反应基因在生长控制中的精确功能尚不清楚，但其中许多可能参与细胞周期中从 G0 到 G1 的转变。核原癌基因junanderbA 的产物是转录因子的研究结果支持了核癌蛋白在基因表达调节中的作用的观点8-11。最近，据报道，FOS 蛋白在转录复合物中与 junoncogene 的产物、转录因子 AP-1 相关（参考文献 12-15）。由于在细胞生长起始期间，fosgene 响应有丝分裂原而被诱导，因此我们研究了核转录因子 AP-1 的表达是否也是可诱导的。我们报道，血清和佛波酯 12-o-十四酰佛波醇 13-乙酸酯 (TPA) 可以快速诱导 mousec-jungene 转录。此外，诱导是短暂的，并且 mRNA 会被蛋白质合成抑制剂过度诱导。
The response of a cell to mitogens and differentiation agents involves the transcriptional induction of several cellular genes. Prominent among these so-called 'immediate early' or 'competence' genes are the nuclear oncogenesfosandmycl–7. Although the precise function of these early response genes in growth control is not understood, it is likely that many of them are involved in the transition from G0to G1in the cell cycle. The findings that the products of nuclear proto-oncogenesjunanderbAare transcriptional factors supports the notion of the role of the nuclear oncoproteins in the regulation of gene expression8–11. Recently, it has been reported that the FOS protein is associated in transcriptional complexes with the product of thejunoncogene, the transcription factor AP-1 (refs 12–15). As thefosgene is induced in response to mitogens during initiation of cell growth, we investigated whether expression of the nuclear transcription factor AP-1 is also inducible. We report that mousec-jungene transcription is rapidly induced by serum and phorbol-ester 12-o-tetradecanoyl phorbol 13-acetate (TPA). Furthermore, induction is transient and the mRNA is superinduced by inhibitors of protein synthesis.