A MONOCLONAL-ANTIBODY (HML-1) DEFINING A NOVEL MEMBRANE MOLECULE PRESENT ON HUMAN INTESTINAL LYMPHOCYTES
A MONOCLONAL-ANTIBODY (HML-1) DEFINING A NOVEL MEMBRANE MOLECULE PRESENT ON HUMAN INTESTINAL LYMPHOCYTES
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DOI：
10.1002/eji.1830170910
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发表时间：
1987-09-01
影响因子：
5.4
通讯作者：
GRISCELLI, C
中科院分区：
文献类型：
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作者：
CERFBENSUSSAN, N;JARRY, A;GRISCELLI, C
单克隆抗体 HML-1 是通过将 NSI 骨髓瘤细胞与用分离的人肠上皮内淋巴细胞 (IEL) 免疫的小鼠的脾细胞融合而产生的。对分离细胞的免疫荧光研究以及组织切片的免疫过氧化物酶染色表明，HML-1 标记了人肠道 IEL 的所有不同亚群，约 40% 的固有层 T 细胞、30% 的肠系膜淋巴细胞和其他粘膜（尤其是 IEL）中的一些淋巴细胞。相反，它只显示了所有其他淋巴室中的稀有细胞。聚丙烯酰胺凝胶梯度电泳分析表明，HML-1 沉淀了大约 mol 的两种主要单共价结合成分。来自人类 IEL 的 105 和 150 kDa 质量。因此，HML-1定义了一种新的人膜抗原，存在于优先与上皮细胞，特别是与肠上皮细胞相关的淋巴细胞亚群上。这种人类抗原的特征与我们之前在大鼠中描述的抗原的特征非常相似。这类新型淋巴细胞膜抗原的可能功能作用以及触发其表达的机制的性质仍有待阐明。
A monoclonal antibody, HML-1, was produced by fusion of NSI myeloma cells wtih spleen cells of a mouse immunized with isolated human intestinal intraepithelial lymphocytes (IEL). Immunofluorescence studies of isolated cells, as well as immunoperoxidase staining of tissue sections, indicated that HML-1 labeled all the various subsets of human intestinal IEL, approximately 40% of lamina propria T cells, 30% mesenteric lymphoblasts and some lymphocytes in other mucosae, particularly IEL. Conversely, it revealed only rare cells in all other lymphoid compartments. Analysis by polyacrylamide gel gradient electrophoresis showed that HML-1 precipitated two major monocovalently bound components of approximate mol. masses of 105 and 150 kDa from human IEL. HML-1 thus defines a novel human membrane antigen present on a subpopulation of lymphocytes preferentially associated with epithelia, and particularly with intestinal epithelium. The characteristics of this human antigen are very similar to those of an antigen we have previously described in the rat. The possible functional role of this novel class of lymphocyte membrane antigens as well as the nature of the mechanism that triggers their expression remains to be elucidated.