Cytokine Array Comparisons of Plasma from Cycling Fertile Women on Cycle Day 5 and Ovulation
Cytokine Array Comparisons of Plasma from Cycling Fertile Women on Cycle Day 5 and Ovulation
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DOI：
10.1111/j.1600-0897.2009.00725.x
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发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Croy, B. Anne
中科院分区：
文献类型：
--
作者：
Hatta, Kota;Bilinski, Michael J.;Croy, B. Anne
问题为了鉴定在人类月经周期的卵泡期和排卵期之间上调或下调的血浆免疫调节分子，研究方法使用RayBio（R）细胞因子阵列来筛选在月经周期第5天的卵泡期和排卵期从5名健康、不吸烟、未使用激素避孕药的育龄妇女。ResultsA共发现23个差异表达的分子：与卵泡期相比，排卵期有10个分子表达上调，13个分子表达下调（α = 0.05，假发现率为0.45）.结论：健康女性的月经周期中，循环免疫调节分子呈波动性变化。差异表达分子的组合表明在血管生成和免疫细胞运输的周期性调节中的作用。
ProblemTo identify plasma immuno-regulatory molecules up or down regulated between the follicular phase and ovulation of the human menstrual cycle.Method of studyRayBio (R) cytokine arrays were used to screen 174 immuno-regulatory molecules in plasma collected during the follicular phase at menstrual cycle day 5 and at ovulation from five healthy, non-smoking, fertile women of reproductive age not using hormonal contraception.ResultsA total of 23 differentially expressed molecules were found: 10 molecules were differentially up-regulated and 13 down-regulated at ovulation compared with that at the follicular phase (alpha = 0.05, false discovery rate of 0.45).ConclusionCirculating immuno-regulatory molecules fluctuate over the menstrual cycle in healthy women. The combination of differentially expressed molecules suggests roles in cyclical regulation of angiogenesis and immune cell trafficking.