Probiotic properties of non-conventional lactic acid bacteria: Immunomodulation by Oenococcus oeni
Probiotic properties of non-conventional lactic acid bacteria: Immunomodulation by Oenococcus oeni
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DOI：
10.1016/j.ijfoodmicro.2010.04.007
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发表时间：
2010-06-15
影响因子：
5.4
通讯作者：
Pot, Bruno
中科院分区：
文献类型：
--
作者：
Foligne, Benoit;Dewulf, Joelle;Pot, Bruno
广泛使用的益生菌属于乳杆菌属（Lactobacillus）和双歧杆菌属（Bifidobacterium），并且在大多数情况下从人胃肠道中分离。然而，其他“不太传统”的细菌，来自异地或极端来源，具有相似的结构或功能特征，也可能赋予宿主特定的健康益处。首先，我们对酒类酒球菌和小片球菌属的25株葡萄酒乳酸菌进行了体外免疫调节或免疫刺激活性的研究。小毕赤酵母菌株刺激外周血单个核细胞（PBMCs）释放的细胞因子变化不大，O. OENI菌株诱导菌株特异性细胞因子模式。一些O。然后在各种生长、剂量和培养基条件下进一步分析OENI菌株。在第二阶段，我们评估了口服耐受性和安全性的两个菌株O。oeni的小鼠喂食高剂量的细菌一周。最后，收集了关于所选O. oeni菌株，使用实验性2，4，6-三硝基苯磺酸（TNBS）诱导的结肠炎小鼠模型。尽管结果与某些常规益生菌获得的抗炎水平不匹配，但菌株IOEB 9115显著降低了结肠损伤并缓解了结肠炎症状。O.对酸、乙醇和酚类化合物的“天然”耐受性。OENI菌株与可测量的免疫调节潜力相结合，表明从葡萄酒中分离的所选菌株作为活益生菌的可能用途。(C)2010 Elsevier B. V.保留所有权利。
The widely used probiotic bacteria belong to the genera Lactobacillus and Bifidobacterium and have in most cases been isolated from the human gastrointestinal tract. However, other "less conventional" bacteria, from allochthonous or extremophilic origin, sharing similar structural or functional features, may also confer specific health benefits to a host. Firstly, we explored the in vitro immuno-modulatory or immune-stimulatory activities of 25 wine lactic acid bacteria belonging to Oenococcus oeni and Pediococcus parvulus. While cytokines released by peripheral blood mononuclear cells (PBMCs) stimulated by P. parvulus strains, showed little variation, O. oeni strains induced strain-specific cytokine patterns. Some O. oeni strains were then further analyzed under various conditions for growth, dose and culture medium. In a second phase, we evaluated the oral tolerance and safety of two strains of O. oeni in mice fed a high dose of bacteria for a week. Finally, evidence was gathered on the in vivo anti-inflammatory potential of a selected O. oeni strain using an experimental 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis mouse model. Although results did not match the anti-inflammatory levels obtained with certain conventional probiotics, strain IOEB 9115 significantly lowered colonic injury and alleviated colitis symptoms. The 'natural' tolerance towards acid, ethanol, and phenolic compounds of O. oeni strains combined with a measureable immunomodulatory potential, suggest a possible use of selected strains isolated from wine as live probiotics. (C) 2010 Elsevier B.V. All rights reserved.