Bisphenol A exposure modifies DNA methylation of imprint genes in mouse fetal germ cells
Bisphenol A exposure modifies DNA methylation of imprint genes in mouse fetal germ cells
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DOI：
10.1007/s11033-012-1716-7
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发表时间：
2012-06
影响因子：
2.8
通讯作者：
Xi-Feng Zhang;Lianjun Zhang;Yanni Feng;Bo Chen;Yan-Min Feng;Gui-Jin Liang;Lan Li;W. Shen
中科院分区：
文献类型：
--
作者：
Xi-Feng Zhang;Lianjun Zhang;Yanni Feng;Bo Chen;Yan-Min Feng;Gui-Jin Liang;Lan Li;W. Shen
双酚A（BPA）是一种雌激素环境毒素，广泛用于塑料生产。人类频繁接触这种化学品被认为是一种潜在的公共健康风险。本研究的目的是评估双酚A对胚胎小鼠生殖细胞中印记基因DNA甲基化的影响。从妊娠后0.5天开始，以0、40、80和160 μg BPA/kg体重/天的剂量对妊娠小鼠进行处理。结果显示，随着BPA浓度的增加，印迹基因Igf 2 r、Peg 3和H19的甲基化水平降低（p< 0.01），BPA处理组雌性生殖细胞中Nobox的相对mRNA水平低于对照组，而BPA处理组雄性生殖细胞中Nobox的相对mRNA水平高于对照组。结果表明，雌胎生殖细胞减数分裂特异性基因包括Stimulated by Stra 8和Dazl的mRNA表达均降低。总之，BPA暴露可以影响胎鼠生殖细胞中印记基因的DNA甲基化。
Bisphenol A (BPA) is an estrogenic environmental toxin widely used for the production of plastics. Human frequent exposure to this chemical has been proposed to be a potential public health risk. The objective of this study was to assess the effects of BPA on DNA methylation of imprinting genes in fetal mouse germ cell. Pregnant mice were treated with BPA at doses of 0, 40, 80 and 160 μg BPA/kg body weight/day from 0.5 day post coitum. DNA methylation of imprinting genes,Igf2r,Peg3andH19, was decreased with the increase of BPA concentration in fetal mouse germ cells (p< 0.01).The relative mRNA levels ofNoboxwere lower in BPA-treated group compared to control (BPA free) in female fetal germ cells, but in male fetal germ cells, a significant higher inNoboxexpression was observed in BPA-treated group compared to control. Decreased mRNA expression of specific meiotic genes including Stimulated byStra8andDazlwere obtained in the female fetal germ cells. In conclusion, BPA exposure can affect the DNA methylation of imprinting genes in fetal mouse germ cells.