Differential expression of transforming growth factor-beta receptors in rat kidney development.
Differential expression of transforming growth factor-beta receptors in rat kidney development.
复制标题
大鼠肾脏发育中转化生长因子-β受体的差异表达。
DOI：
10.1152/ajprenal.1997.273.3.f386
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Ballermann,BJ
中科院分区：
文献类型：
--
作者：
Choi,ME;Liu,A;Ballermann,BJ
转化生长因子-β 1（TGF-β 1）在胚胎发生过程中以及在经历强烈发育和形态发生的部位强烈表达。两种受体丝氨酸/苏氨酸激酶（I型和II型）已被鉴定为信号转导TGF-β受体。本研究旨在进一步探索不同TGF-β受体在肾脏发育过程中的作用。寻找大鼠中两种T β R-I（即激活素受体样激酶-5（ALK-5）和Tsk 7 L）的物种特异性序列信息。从新生大鼠肾和肺文库中克隆了两个全长T β R-I cDNA，测序显示它们是人ALK-5和小鼠Tsk 7 L的大鼠同源物。通过北方印迹分析测定，I型和II型TGF-β受体均在肾脏中表达。与成年大鼠肾脏相比，新生大鼠肾脏中的T β R-II mRNA丰度显著更高。同样，ALK-5 mRNA在胎儿和新生大鼠肾脏中的表达高于成年大鼠肾脏。与此相反，Tsk 7 L mRNA丰度之间的胎儿，新生儿和成年大鼠肾脏没有显着差异。因此，基于这些发现，T β R-II和ALK-5在肾脏中受到发育调节。通过免疫组织化学证实了发育中的肾脏中T β R-II和ALK-5蛋白的表达增加。有趣的是，这两种TGF-β受体并不完全共定位，这增加了其他TGF-β信号受体可能参与的有趣可能性。
Transforming growth factor-beta 1 (TGF-beta 1) is strongly expressed during embryogenesis and in sites undergoing intense development and morphogenesis. Two receptor serine/threonine kinases (types I and II) have been identified as signal-transducing TGF-beta receptors. This study was undertaken to further explore the role of the distinct TGF-beta receptors during kidney development. The species-specific sequence information for the two T beta R-I, namely, activin receptor-like kinase-5 (ALK-5) and Tsk7L, in the rat was sought. Two full-length T beta R-I cDNAs were cloned from a neonatal rat kidney and lung libraries, and sequencing revealed that they were the rat homologs of human ALK-5 and murine Tsk7L. Both types I and II TGF-beta receptors are expressed in the kidney as determined by Northern blot analysis. T beta R-II mRNA abundance was significantly greater in the neonatal rat kidney compared with the adult rat kidney. Similarly, ALK-5 mRNA was more highly expressed in the fetal and neonatal rat kidney than the adult rat kidney. In contrast, there was no significant difference in Tsk7L mRNA abundance among the fetal, neonatal, and adult rat kidney. Thus, based on these findings, both T beta R-II and ALK-5 are developmentally regulated in the kidney. Increased expression of T beta R-II and ALK-5 proteins in the developing kidney was confirmed by immunohistochemistry. Interestingly, the two TGF-beta receptors did not entirely colocalize, raising the intriguing possibility that other TGF-beta signaling receptors may be involved.