Sex differences in androgen receptors and aromatase activity in microdissected regions of the rat brain.
Sex differences in androgen receptors and aromatase activity in microdissected regions of the rat brain.
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DOI：
10.1210/endo-128-3-1310
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发表时间：
1991-03
期刊：
影响因子：
4.8
通讯作者：
C. Roselli
中科院分区：
文献类型：
--
作者：
C. Roselli
男性通常比女性对雄激素的行为和神经内分泌作用更敏感。本实验是为了确定这些差异是否可能导致性别差异的雄激素受体（AR）的数量在特定的脑区。出于这个原因，比较了用产生相等生理循环雄激素水平的睾酮（T）剂量处理的性腺切除雄性和雌性大鼠的显微解剖脑区的胞质溶胶（ARc）和细胞核KCl提取物（ARn）中的AR结合。此外，微粒体芳香化酶活性测定作为组织对T反应的生化指标，因为某些脑区的雌激素形成受雄激素调节。外源性T给药后一周，男性表现出显着较高水平的ARn比女性在床核的终纹，脑室周围视前区，腹内侧核。男性也有显着较高的芳香化酶水平在这些相同的领域加上内侧视前核和下丘脑前部。在这些实验条件下观察到的ARc水平的显着性别差异进行了检查，在其他八个核的ARn浓度没有显着差异。当在未处理的性腺切除雄性和雌性大鼠中比较ARc水平时，雄性大鼠仅在终纹床核中的ARc水平较高，表明新受体合成可能是T处理大鼠中观察到的性别差异的原因。这些结果表明，在神经反应雄激素的性别差异可能是由于部分的ARn占领在特定的大脑区域的性别差异。
Males are generally more responsive than females to the behavioral and neuroendocrine actions of androgens. The present experiments were performed to determine whether these differences may result from sex differences in the number of androgen receptors (AR) in specific brain areas. For this reason, AR binding was compared in both cytosol (ARc) and cell nuclear KCl extracts (ARn) from microdissected brain regions of gonadectomized male and female rats treated with doses of testosterone (T) that produced equivalent physiological circulating androgen levels. In addition, microsomal aromatase activity was measured as a biochemical index of tissue responsiveness to T, since estrogen formation in certain brain areas is regulated by androgen. One week after exogenous T administration, males exhibited significantly higher levels of ARn than females in the bed nucleus of the stria terminalis, periventricular preoptic area, and ventromedial nucleus. Males also had significantly higher aromatase levels in these same areas plus the medial preoptic nucleus and anterior hypothalamus. There were no significant differences in ARn concentrations in eight other nuclei that were examined for significant sex differences in ARc levels observed under these experimental conditions. When ARc levels were compared in untreated gonadectomized male and female rats, males had greater levels of ARc in the bed nucleus of the stria terminalis only, indicating that new receptor synthesis may be responsible for the sex differences observed in T-treated rats. These results suggest that sex differences in neural responsiveness to androgens may be due in part to sex differences in ARn occupation in specific brain regions.