Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins
Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins
复制标题
以图案化多孔硅为模板的凝胶垫微阵列，用于蛋白质的双模式检测
DOI：
10.1039/b821265a
复制
发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Jian-Ning
中科院分区：
文献类型：
--
作者：
Chen, Ling;Chen, Zeng-Tai;Liu, Jian-Ning
概念验证研究通过引发（聚乙二醇）甲基丙烯酸酯（PEGMA）与表面 Si-H 物质的原子转移自由基传播（ATRP）聚合、凝胶膜逐步化学转化为 NTA-Ni2+/组氨酸标记的蛋白质系统以及基质辅助激光解吸/电离质谱（MALDI MS）和荧光检测，证明了多孔硅芯片上新型凝胶垫微阵列的可行性。
A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation ( ATRP) polymerisation of ( polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.