REGULATION OF ANTENNAPEDIA TRANSCRIPT DISTRIBUTION BY THE BITHORAX COMPLEX IN DROSOPHILA
REGULATION OF ANTENNAPEDIA TRANSCRIPT DISTRIBUTION BY THE BITHORAX COMPLEX IN DROSOPHILA
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DOI：
10.1038/307287a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
--
作者：
HAFEN, E;LEVINE, M;GEHRING, WJ
同源异型基因ofDrosophilaseem参与发育途径的建立和不同解剖节身份的特化1，2。例如，已经提出，胸腺+（Antp+）基因座的功能是指导胚胎细胞遵循中胸发育途径3 -5。使用克隆的Antp+（参考文献6）基因座的cDNA探针和改进的原位杂交方法7，我们发现胚胎和幼虫中胸的神经细胞比其他节段的神经组织具有更高水平的Antp+转录本7，8。在这里，我们提出了进一步的证据表明，通过分析双胸突变体中Antp+转录本的分布，Antp+产物与中胸的形成有关。任何一个双胸突变的纯合子胚胎显示更后节的表皮转化为间胸的同源组织1。例如，后胸和前七个腹部节段的胚胎，完全缺乏thebithorax基因复合体显示表皮表型特征的正常间胸1，9。通过Antp ~+cDNA探针与这些胚胎组织切片的原位杂交，我们发现转化节段的神经细胞积累Antp ~+转录本达到正常间胸的特征水平。
The homoeotic genes ofDrosophilaseem to be involved in the establishment of developmental pathways and the specification of different anatomical segment identities1,2. For example, it has been proposed that a function of theAntennapedia+(Antp+) locus is to direct embryonic cells to follow a mesothoracic developmental pathway3–5. Using cloned cDNA probes of theAntp+(ref. 6) locus and an improvedin situhybridization method7, we found that the neural cells of the embryonic and larval mesothorax possess higher levels ofAntp+transcripts than the neural tissue of the other segments7,8. Here we present further evidence thatAntp+products are associated with formation of the mesothorax, obtained by analysing the distribution ofAntp+transcripts inbithoraxmutants. Embryos homozygous for any one of severalbithoraxmutations display a transformation of the epidermis of the more posterior segments into the homologous tissue of the mesothorax1. For example, the metathorax and first seven abdominal segments of embryos that completely lack thebithoraxgene complex show a cuticular phenotype characteristic of the normal mesothorax1,9. Byin situhybridization of anAntp+cDNA probe to tissue sections of these embryos, we have found that the neural cells of the transformed segments accumulateAntp+transcripts to a level characteristic of the normal mesothorax.