Self-assembly of double-stranded DNA molecules at nanomolar concentrations
Self-assembly of double-stranded DNA molecules at nanomolar concentrations
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DOI：
10.1021/bi061539y
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发表时间：
2007-01-09
期刊：
影响因子：
2.9
通讯作者：
Ohyama, Takashi
中科院分区：
文献类型：
--
作者：
Inoue, Shotaro;Sugiyama, Shigeru;Ohyama, Takashi
一些蛋白质具有自组装的性质，已知是构建细胞功能的超分子结构的重要机制。然而，到目前为止，双链DNA分子自组装的能力还没有建立。在这里，我们报告说，dsDNA分子也有一个属性的自组装在含有生理浓度的Mg 2+的水溶液中。我们表明，DNA分子优先相互作用的分子具有相同的序列和长度，即使在溶液中组成的异质性DNA物种。弯曲的DNA和具有不寻常构象和性质的DNA也表现出这种现象，表明它不是通常的B型DNA所特有的。原子力显微镜（AFM）直接揭示了组装的DNA分子形成的浓度为10 nM，但很少在1 nM。自组装是浓度依赖性的。我们认为，吸引力引起的DNA自组装可能在生物过程中发挥作用，如重复DNA的折叠，同源序列之间的重组，以及减数分裂中的突触。
Some proteins have the property of self-assembly, known to be an important mechanism in constructing supramolecular architectures for cellular functions. However, as yet, the ability of double-stranded (ds) DNA molecules to self-assemble has not been established. Here we report that dsDNA molecules also have a property of self-assembly in aqueous solutions containing physiological concentrations of Mg2+. We show that DNA molecules preferentially interact with molecules with an identical sequence and length even in a solution composed of heterogeneous DNA species. Curved DNA and DNA with an unusual conformation and property also exhibit this phenomenon, indicating that it is not specific to usual B-form DNA. Atomic force microscopy (AFM) directly reveals the assembled DNA molecules formed at concentrations of 10 nM but rarely at 1 nM. The self-assembly is concentration-dependent. We suggest that the attractive force causing DNA self-assembly may function in biological processes such as folding of repetitive DNA, recombination between homologous sequences, and synapsis in meiosis.