Neurite guidance by the FnC repeat of human tenascin-C: neurite attraction vs. neurite retention.
Neurite guidance by the FnC repeat of human tenascin-C: neurite attraction vs. neurite retention.
复制标题
人腱蛋白-C 的 FnC 重复的神经突引导：神经突吸引与神经突保留。
DOI：
10.1111/j.1460-9568.2005.04383.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Meiners,Sally
中科院分区：
文献类型：
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作者：
Liu,Hsing-Yin;Nur-E-Kamal,Alam;Schachner,Melitta;Meiners,Sally
人腱生蛋白C（fnC）的可变剪接的纤连蛋白III型重复序列C为体外延长轴突提供了方向性线索。当在与聚赖氨酸（PLL）的界面处给予选择时，大鼠小脑颗粒神经突优先交叉到fnC上（本文定义为神经突吸引），而源自fnC的神经突优先保留在fnC上（定义为神经突保留）。使用跨越fnC序列的合成肽进一步精制指导基序。我们发现具有氨基酸序列DINPYGFTVSWMASE的肽足以吸引和保留神经突。肽与NPYG的改变促进神经突起的保留，但不吸引，相反，分子与ASE的改变促进神经突起的吸引，但不保留。因此，由fnC介导的神经突吸引和神经突保留是可以独立调节的可分离事件。这种性质可能证明有价值的战略设计的肽试剂用于战略，以促进定向轴突再生后中枢神经系统损伤。
The alternatively spliced fibronectin type‐III repeat C of human tenascin‐C (fnC) provides directional cues to elongating neuritesin vitro. When given a choice at an interface with polyl‐lysine (PLL), rat cerebellar granule neurites preferentially crossed onto fnC (defined herein as neurite attraction) whereas neurites originating on fnC preferentially remained on fnC (defined as neurite retention). Guidance motifs were further refined using synthetic peptides spanning the sequence of fnC. We found that a peptide with amino acid sequence DINPYGFTVSWMASE was sufficient to attract and retain neurites. Peptides with alterations in NPYG facilitated neurite retention but not attraction and, conversely, molecules with alterations in ASE facilitated neurite attraction but not retention. Hence neurite attraction and neurite retention mediated by fnC are separable events that can be independently regulated. This property may prove valuable for the strategic design of peptide reagents for use in strategies to facilitate directed axonal regrowth following CNS injury.