A convenient purfication method for metal nanoclusters based on pH-induced aggregation and cyclic regeneration and its applications in fluorescent pH sensors
A convenient purfication method for metal nanoclusters based on pH-induced aggregation and cyclic regeneration and its applications in fluorescent pH sensors
复制标题
基于pH诱导聚集和循环再生的金属纳米团簇便捷纯化方法及其在荧光pH传感器中的应用
DOI：
10.1016/j.snb.2016.08.114
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wang Shengfu
中科院分区：
文献类型：
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作者：
Xiong Huayu;Wang Wei;Liang Jichao;Wen Wei;Zhang Xiuhua;Wang Shengfu
在此，我们报告了一种方便的方法来纯化水溶性牛血清白蛋白（BSA）的覆盖金属纳米团簇的pH诱导沉淀在酸性条件下。当纳米颗粒重新分散到碱性介质中时，金属纳米团簇的荧光可以完全恢复。BSA金属纳米团簇已经被制备用于在高酸性介质中监测pH。当pH降低时，在UV灯下纳米团簇的荧光降低，并且在pH 4之后完全消失。基于金团簇琼脂糖凝胶的“OFF-ON”信号开关，研制了一种用于pH监测的荧光pH传感器。
Herein, we report a convenient approach to purify water-soluble bovine serum albumin (BSA)-capped metal nanoclusters by pH-induced precipitation under acidic conditions. The fluorescence of metal nanoclusters could be completely recovered upon the re-dispersion of nanoparticles into basic media. BSA metal nanoclusters have been prepared to monitor pH at highly acidic medium. When pH decreased, the fluorescence of the nanoclusters under a UV lamp decreased and completely disappeared after pH 4. And a fluorescent pH sensor for pH monitoring has been developed based on the “OFF–ON” signal switch of the gold clusters agarose hydrogel.