Ecological study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at sub-sites from approximal dental plaque from children
Ecological study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at sub-sites from approximal dental plaque from children
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DOI：
10.1159/000016430
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发表时间：
1998-01-01
期刊：
影响因子：
4.2
通讯作者：
Newman, HN
中科院分区：
文献类型：
--
作者：
Babaahmady, KG;Challacombe, SJ;Newman, HN
先前的免疫荧光（IF）研究表明，变形链球菌可能优先定植在近似斑块内的特定亚位点。本研究旨在将这些观察结果扩展到龈缘菌斑中的其他变异链球菌和乳酸菌。从64名儿童的90颗牙齿（每颗牙齿3颗）中提取270个近似菌斑样本；与接触区相关的三个龈缘亚位：远离(A)、靠近(S)和低于(B)接触区。样品采用高滴度多克隆抗s间接IF处理。变‘c’，反s。sobrinus 'd'，反l。casei和anti-L。嗜酸的抗血清。S. mutans ‘c’与S.sobrinus ‘d’呈显著正相关（p < 0.05）。乳酸菌很少被分离出来，通常与多发性硬化症一起被发现。乳酸菌或多发性硬化症的存在与龋齿（仅为白斑病变）呈正相关，尽管这些物种通常可以从非龋齿部位分离出来。突变链球菌‘c’和sobrinus ‘d’的存在与早期龋齿病变密切相关。此外，本研究还证实了牙菌斑周围不同亚位点的菌群差异。
Previous immunofluorescence (IF) studies have indicated that Streptococcus mutans may preferentially colonise specific sub-sites within approximal plaque. The present study aimed to extend these observations to other mutans streptococci and lactobacilli in such gingival margin plaque. Two hundred and seventy approximal plaque samples were taken from 90 teeth (3 from each tooth) in 64 children; three gingival margin sub-sites in relation to the contact area: away from (A), to the side of (S) and below (B) the contact area. Samples were processed by indirect IF using high-titred polyclonal anti-S. mutans 'c', anti-S. sobrinus 'd', anti-L. casei and anti-L. acidophilus antisera. An overall positive association was found between S. mutans 'c' and S.sobrinus 'd' (p S, p < 0.05). Lactobacillus spp. were isolated rarely, and were usually found together with MS. There was a positive relationship between the presence of lactobacilli or MS and caries (white spot lesions only), although these species could frequently be isolated from non-carious sites. The presence of both S. mutans 'c' and S. sobrinus 'd' were strongly correlated with early caries lesions. In addition, this study confirmed the variation in the microflora at different sub-sites within approximal dental plaque.