Transcriptome analysis revealed key genes involved in flavonoid metabolism in response to jasmonic acid in pigeon pea (Cajanus cajan (L.) Millsp.)
Transcriptome analysis revealed key genes involved in flavonoid metabolism in response to jasmonic acid in pigeon pea (Cajanus cajan (L.) Millsp.)
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转录组分析揭示了木豆（Cajanus cajan (L.) Millsp.）中茉莉酸参与类黄酮代谢的关键基因
DOI：
10.1016/j.plaphy.2021.10.022
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发表时间：
2021-10-27
影响因子：
6.5
通讯作者：
Fu, Yujie
中科院分区：
文献类型：
--
作者：
Du, Tingting;Fan, Yuxin;Fu, Yujie
黄酮类化合物是木豆重要的代谢产物，与木豆的抗逆能力有关。然而，木豆黄酮类化合物的分子基础和调控机制尚不清楚。茉莉酸甲酯（MeJA）是一种与类黄酮生物合成相关的信号分子。外源10 mg/ L茉莉酸甲酯（MeJA）处理木豆后，可减轻木豆病原真菌的侵染，提高木豆中黄酮类化合物的含量。分别通过转录组测序和高效液相色谱分析基因表达和代谢变化的结果表明：（1）染料木素、芹菜素、牡荆素和鹰嘴豆芽素A等多种黄酮类化合物的浓度显著上调;（2）产生了13675个差异表达基因，主要集中在信号转导和生物合成途径：（3）类黄酮合成途径中的关键合成酶基因（CcI 2 ' H、CcHIDH、Cc 7-IOMT）的表达水平显著上调;（4）CcbHLH 35的过表达显著上调类黄酮合成酶基因的表达，并诱导染料木素、牡荆素和芹菜素的积累。本研究揭示了MeJA在木豆类黄酮合成和功能中的重要作用，为进一步研究木豆类黄酮介导的防御反应奠定了基础。
Flavonoids are important metabolites of pigeon pea in relation to its stress resistance. However, the molecular basis and regulatory mechanisms of flavonoids in pigeon pea remain unclear. Methyl jasmonate (MeJA) is a signaling molecule associated with biosynthesis of flavonoids. In this study, after exogenous treatment of 10 mg/ L MeJA, infection of pathogenic fungi to pigeon pea was alleviated and the content of flavonoids was increased. Results of gene expression and metabolic changes that were respectively analyzed by transcriptome sequencing and high performance liquid chromatography (HPLC) showed that (1) concentrations of various flavonoids, such as genistein, apigenin, vitexin and biochanin A were significantly up-regulated; (2) 13675 differentially expressed genes were produced, mainly enriched in signal transduction and isoflavone biosynthesis pathways: (3) the expression levels of key synthase genes (CcI2 ' H, CcHIDH, Cc7-IOMT) in the flavonoid biosynthesis pathway were significantly up-regulated; (4) Overexpression of CcbHLH35 significantly induced upregulation of flavonoid synthase genes and accumulation of genistein, vitexin and apigenin. Our findings reveals the pivotal roles of MeJA in synthesis and functioning of flavonoids in pigeon pea, which provide a basis for further studies on flavonoid-mediated defense responses.