A Putative MerR Family Regulator Involved in Biofilm Formation in Listeria monocytogenes 4b G
A Putative MerR Family Regulator Involved in Biofilm Formation in Listeria monocytogenes 4b G
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假定的 MerR 家族调节因子参与单核细胞增生李斯特菌 4b G 生物膜的形成
DOI：
10.1089/fpd.2011.1101
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发表时间：
2012-08-01
影响因子：
2.8
通讯作者：
Shi, Xianming
中科院分区：
文献类型：
--
作者：
Huang, Yanyan;Shi, Chunlei;Shi, Xianming
单增李斯特菌形成生物膜的能力使其对环境胁迫具有更强的抵抗力。为了研究生物膜形成的机理，L。本研究表征了具有降低的生物膜形成能力的单核细胞增多症转座子突变株GB8。Southern杂交结果显示，转座子在GB8染色体上有一个单拷贝，反向聚合酶链反应（IPCR）鉴定了插入位点。lm的表情。G_1497基因在转座子插入后失活，与已测序的LMOf2365_1497基因完全一致。单核细胞增生菌株4b F2365，编码MerR家族蛋白。回复突变株GBR 8恢复了生物被膜形成能力。通过实时荧光定量PCR证实lm基因侧翼的转录。G_1497不受Tn917插入的影响。这些结果表明，lm.G_1497对L.单核细胞增多症4b G.此外，lm.G_1497表达失败导致自溶率下降，而对L.单核细胞增多症GB8，这可能意味着生物膜的减少是由细胞自溶的差异引起的。
The capability of forming biofilm makes the foodborne pathogen Listeria monocytogenes more resistant to environmental stresses. To examine the mechanism of biofilm formation, L. monocytogenes transposon mutant strain GB8 with decreased ability to form biofilm was characterized in this study. Southern blot assay revealed a single copy of transposon in the GB8 chromosome, and the insertion site was identified by inverse polymerase chain reaction (IPCR). The expression of lm. G_1497 was found to be inactivated by the transposon insertion, and the gene was identical to gene LMOf2365_1497 in the sequenced strain L. monocytogenes strain 4b F2365, encoding a MerR family protein. The ability of producing biofilm was recovered in revertant GBR8. It was confirmed by quantitative real-time PCR that the transcription of the gene flanking lm. G_1497 was not affected by Tn917 insertion. These results suggested that lm.G_1497 was responsible for the positive regulation of biofilm formation in L. monocytogenes 4b G. Moreover, the failure of lm.G_1497 expression resulted in a decrease of the autolysis rate, while no effect on cell growth and motility in L. monocytogenes GB8, which might imply that the reduction in biofilm was caused by the difference in cell autolysis.