Array and distribution of actin filaments in guard cells contribute to the determination of stomatal aperture
Array and distribution of actin filaments in guard cells contribute to the determination of stomatal aperture
复制标题
保卫细胞中肌动蛋白丝的阵列和分布有助于气孔孔径的确定
DOI：
10.1007/s00299-008-0581-2
复制
发表时间：
2008-10-01
影响因子：
6.2
通讯作者：
Wang, Xue-Chen
中科院分区：
文献类型：
--
作者：
Gao, Xin-Qi;Chen, Jing;Wang, Xue-Chen
保卫细胞中的肌动蛋白细丝及其在调节气孔运动中的动力学功能。本研究采用蚕豆中微量注射Alexa-phalloidin和烟草中表达GFP-mTn2种重要标记技术，研究了蚕豆气孔运动过程中保卫细胞内肌动蛋白细丝的排列和分布。我们发现，大多数关闭的气孔中的肌动蛋白细丝的随机排列在气孔开放后变成了环状排列。在开放气孔(皮质分布)中，肌动蛋白细丝主要分布在皮质细胞质中，分布均匀，分布于闭合气孔保卫细胞的胞浆内。这些结果表明，保卫细胞中肌动蛋白细丝的随机排列和均匀分布可能是维持关闭的气孔所必需的；同样，肌动蛋白细丝的环状排列和皮质分布也对维持开放的气孔起作用。此外，我们还发现，肌动蛋白解聚这一气孔运动的特征，促进了肌动蛋白的排列和分布随气孔运动的变化。因此，肌动蛋白细丝的解聚有利于保卫细胞肌动蛋白排列和分布的改变，从而促进气孔运动。
Actin filaments in guard cells and their dynamics function in regulating stomatal movement. In this study, the array and distribution of actin filaments in guard cells during stomatal movement were studied with two vital labeling, microinjection of alexa-phalloidin inVicia fabaand expression ofGFP-mTnin tobacco. We found that the random array of actin filaments in the most of the closed stomata changed to a ring-like array after stomatal open. And actin filaments, which were throughout the cytoplasm of guard cells of closed stomata (even distribution), were mainly found in the cortical cytoplasm in the case of open stomata (cortical distribution). These results revealed that the random array and even distribution of actin filaments in guard cells may be required for keeping the closed stomata; similarly, the ring-like array and cortical distribution of actin filaments function in sustaining open stomata. Furthermore, we found that actin depolymerization, the trait of moving stomata, facilitates the transformation of actin array and distribution with stomatal movement. So, the depolymerization of actin filaments was favorable for the changes of actin array and distribution in guard cells and thus facilitated stomatal movement.