Identification and expression of a new type II transmembrane protein in human mast cells
Identification and expression of a new type II transmembrane protein in human mast cells
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DOI：
10.1016/j.ygeno.2005.03.006
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发表时间：
2005-07-01
期刊：
影响因子：
4.4
通讯作者：
Yao, ZB
中科院分区：
文献类型：
--
作者：
Li, K;Wang, SW;Yao, ZB
通过消减克隆从人类肥大细胞中分离出编码新型 II 型跨膜蛋白的 cDNA。该 cDNA 包含 186 个氨基酸的开放阅读框。 RT-PCR分析表明该基因在肥大细胞中差异表达。因此，该基因编码的肽被称为肥大细胞表达的膜蛋白1（MCEMP1）。 MCEMP1 基因包含七个外显子，定位于人类染色体 19p13.3。表位标记的 MCEMP1 已在哺乳动物细胞中表达，并被发现定位于细胞膜，其 C 端延伸至膜外，N 端延伸至细胞质区室。生成抗 MCEMP1 的单克隆抗体，并通过免疫沉淀和 FACS 进行表征。结果表明，天然 MCEMP1 在脐带血来源的肥大细胞以及 HMGI 和 THP-1 细胞系中表达，但在我们测试的其他细胞类型中不表达。人肺切片的免疫化学染色表明 MCEMP1 染色与肺肥大细胞特异性相关。 (c) 2005 Elsevier Inc. 保留所有权利。
A cDNA encoding a new type II transmembrane protein has been isolated from human mast cells by subtraction cloning. This cDNA contains an open reading frame of 186 amino acids. RT-PCR analysis showed that this gene is differentially expressed in mast cells. Therefore, the peptide encoded by this gene was termed mast cell-expressed membrane protein 1 (MCEMP1). The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. Monoclonal antibodies against MCEMP1 were generated and characterized by immunoprecipitation and FACS. The results showed that the native MCEMP1 is expressed in cord blood-derived mast cells and HMGI and THP-1 cell lines, but not in other cell types that we have tested. Immunochemical staining of human lung sections showed that MCEMP1 staining is specifically associated with lung mast cells. (c) 2005 Elsevier Inc. All rights reserved.