In vivo exposure of female rats to toxicants may affect oocyte quality
In vivo exposure of female rats to toxicants may affect oocyte quality
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DOI：
10.1016/s0890-6238(03)00009-1
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发表时间：
2003-05-01
影响因子：
3.3
通讯作者：
Horner, CM
中科院分区：
文献类型：
--
作者：
Berger, T;Horner, CM
雌性生殖毒物的一个潜在终点是卵母细胞的受精能力。尚未对哺乳动物雌性动物的该终点进行充分检查。这些研究的目的是评价大鼠卵母细胞在体内暴露于已知的雄性生殖毒物（通过不包括内分泌干扰的途径发挥作用）和已知干扰早期卵泡发育的4-乙烯基环己烯二环氧化物后的受精能力。从暴露后的雌性获得卵母细胞，并通过体外受精率评估质量。一项研究评估了雌性动物暴露于吸入性四氯乙烯2周（2小时/天，5天/周）后的受精能力。其余的研究评估了通过饮用水暴露于四氯乙烯、三氯乙烯、燃料氧化剂甲基叔丁基醚（MTBE）、乙基叔丁基醚（ETBE）、叔戊基甲基醚（驯服）和前两种醚的代谢物2-甲基-1，2-丙二醇（2 M2 P）以及4-乙烯基环己烯二环氧化物2周后立即进行的受精能力。在吸入暴露于四氯乙烯或消耗三氯乙烯或驯服后，受精卵母细胞的百分比降低。受精能力没有改变暴露于其他生殖毒物或其他燃料氧化剂。与暴露于三氯乙烯后卵母细胞受精能力降低一致，暴露女性的卵母细胞结合精子质膜蛋白的能力降低。女性生殖能力评估的终点，卵母细胞的受精能力，降低暴露于三氯乙烯和吸入四氯乙烯。(C)2003年爱思唯尔科学公司All rights reserved.
A potential endpoint for female reproductive toxicants is fertilizability of the oocytes. This endpoint has not been adequately examined for mammalian females. The objective of these studies was to evaluate fertilizability of rat oocytes following in vivo exposure to known male reproductive toxicants that exert effects via pathways that do not include endocrine disruption and to 4-vinylcyclohexene diepoxide, known to interfere with early follicular development. Oocytes were obtained from females following exposure and quality assessed by in vitro fertilization rate. One study evaluated fertilizability following 2 weeks exposure of females to inhaled tetrachloroethylene (2 h/day, 5 days/week). The remaining studies evaluated fertilizability immediately following 2 weeks exposure via drinking water to tetrachloroethylene, trichloroethylene, the fuel oxidants methyl tertiary butyl ether (MTBE), ethyl tertiary butyl ether (ETBE), tertiary amyl methyl ether (TAME), and a metabolite of the first two ethers 2-methyl-1,2-propanediol (2M2P), and to 4-vinylcyclohexene diepoxide. The percentage of oocytes fertilized was reduced following inhalation exposure to tetrachloroethylene, or consumption of trichloroethylene or TAME. Fertilizability was not altered by exposures to the other reproductive toxicants or to the other fuel oxidants. Consistent with the reduced oocyte fertilizability following exposure to trichloroethylene, oocytes from exposed females had a reduced ability to bind sperm plasma membrane proteins. Female reproductive capability assessed by the endpoint, oocyte fertilizability, was reduced by exposure to trichloroethylene and inhaled tetrachloroethylene. (C) 2003 Elsevier Science Inc. All rights reserved.