The roles of grouper clathrin light chains in regulating the infection of a novel marine DNA virus, Singapore grouper iridovirus
The roles of grouper clathrin light chains in regulating the infection of a novel marine DNA virus, Singapore grouper iridovirus
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石斑鱼网格蛋白轻链在调节新型海洋 DNA 病毒新加坡石斑鱼虹彩病毒感染中的作用
DOI：
10.1038/s41598-019-51725-5
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发表时间：
2019-10
影响因子：
4.6
通讯作者：
Qin Q
中科院分区：
文献类型：
--
作者：
Wang L;Li Q;Ni S;Huang Y;Wei J;Liu J;Yu Y;Wang S;Qin Q
网状蛋白由网状蛋白重链(CHC)和网状蛋白轻链(CLCs)组成，通常被病毒劫持以进行感染。然而，CLCs的作用，特别是在调节鱼类病毒感染方面的作用，仍然知之甚少。在本研究中，从赤点石斑鱼(Epinephelus Akaara)中克隆了两种CLCs亚型(EaCLCa和EaCLCb)。两种EaCLC转录本在所有受检组织中均有表达，且EaCLCa的表达水平明显高于EaCLCb。过表达EaCLCA-W119R突变体显著降低新加坡石斑鱼虹彩病毒(SGIV)的感染力。而EaCLCb-W122R对SGIV感染无明显影响。详细的步骤进行了进一步的研究，主要包括病毒的附着、进入和随后的转运到早期内吞体内。EaCLCA-W119R突变体显著抑制SGIV颗粒的内化，但对SGIV附着无影响。此外，EaCLCA-W119R突变体在病毒内化后明显损害了SGIV向早期内吞体内的递送。此外，EaCLCa-W119R突变体显著降低了SGIV和肌动蛋白的共定位。然而，在SGIV感染期间，这种事件不需要EaCLCb。综上所述，这些结果首次证明了EaCLCa和EaCLCb对虹膜病毒感染的不同影响，为更好地了解SGIV感染的机制提供了机会，并为设计新的抗病毒策略提供了机会。
Clathrins, composed of clathrin heavy chains (CHCs) and clathrin light chains (CLCs), are usually hijacked by viruses for infection. However, the role of CLCs, especially in regulating fish virus infection, remains poorly understood. Here, two isoforms of CLCs were cloned from the red-spotted grouper (Epinephelus akaara) (EaCLCa and EaCLCb). Both EaCLC transcripts were expressed in all examined tissues, and the expression of EaCLCa was much higher than that of EaCLCb. Over-expressing EaCLCa-W119R mutant significantly reduced Singapore grouper iridovirus (SGIV) infectivity. However, no effect of EaCLCb-W122R on SGIV infection was observed. The detailed steps were further studied, mainly including virus attachment, entry and the following transport to early endosomes. EaCLCa-W119R mutant notably inhibited internalization of SGIV particles with no effect on SGIV attachment. Furthermore, EaCLCa-W119R mutant obviously impaired the delivery of SGIV to early endosomes after virus internalization. In addition, the EaCLCa-W119R mutant markedly reduced the colocalization of SGIV and actin. However, EaCLCb is not required for such events during SGIV infection. Taken together, these results demonstrate for the first time that EaCLCa and EaCLCb exerted different impacts on iridovirus infection, providing a better understanding of the mechanisms of SGIV infection and opportunities for the design of new antiviral strategies.
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DOI：
10.1016/s0021-9258(18)98957-x
发表时间：
1991-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Ungewickell;H. Ungewickell
通讯作者：
E. Ungewickell;H. Ungewickell
DOI：
10.1093/emboj/17.5.1297
发表时间：
1998-03
期刊：
The EMBO Journal
影响因子：
--
作者：
J. Ybe;B. Greene;Shu-Hui Liu;Ursula M. Pley;P. Parham;F. Brodsky
通讯作者：
J. Ybe;B. Greene;Shu-Hui Liu;Ursula M. Pley;P. Parham;F. Brodsky
影响因子：
4.5
作者：
Huang, Xiaohong;Huang, Youhua;Qin, Qiwei
通讯作者：
Qin, Qiwei
影响因子：
9.2
作者：
Ferreira, Filipe;Foley, Matthew;Smythe, Elizabeth
通讯作者：
Smythe, Elizabeth
影响因子：
5.4
作者：
Huang, Cheng;Chang, Shin C.;Chang, Ming-Fu
通讯作者：
Chang, Ming-Fu