Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation
Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation
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DOI：
10.1292/jvms.12-0141
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发表时间：
2013-01-01
影响因子：
1.2
通讯作者：
Watanabe, Daisaku
中科院分区：
文献类型：
--
作者：
Ishida, Sakura;Yonezawa, Tomohiro;Watanabe, Daisaku
日本黑牛在营养摄入充足的情况下，偶尔也会表现出生长迟缓。为了阐明激素和转录特征，我们研究了6头生长迟缓牛（GR牛）和8头相同年龄、血统、生长正常的对照牛血液成分（包括激素）的差异，以及肝脏和外周血淋巴细胞穷尽基因表达的差异。GR牛红细胞压积值及血红蛋白、血清白蛋白、总胆固醇、胰岛素样生长因子1 （IGF-1）、甲状腺素和胰岛素浓度均显著低于对照组。转基因牛的生长激素分泌水平也较高。我们用3头GR牛和3头对照牛进行肝脏微阵列分析，发现279个基因表达有显著差异。然而，与GH-IGF-1轴相关的基因表达，如GH受体和IGF-1，与对照组没有显著差异。免疫相关基因表达明显降低。为了明确这些基因的表达水平，我们使用外周血淋巴细胞进行实时RT-PCR。肝脏中趋化因子配体8、干扰素受体1和免疫球蛋白轻链VJ区等显著降低的基因在3头GR牛中的表达率也显著低于3头对照牛。这些结果表明，本研究中生长迟缓的原因是其他因素，而不是肝脏GH-IGF-1轴相关因子的基因表达异常，并且GR牛易患传染病。
Japanese Black cattle occasionally demonstrate growth retardation despite sufficient nutrient intake. To clarify hormonal and transcriptional characteristics, we investigated differences in blood components, including hormones, and differences in exhaustive gene expressions in the liver and peripheral lymphocytes of six cattle with growth retardation (GR cattle) and eight control cattle of the same age and pedigree with normal growth. Hematocrit values and concentrations of hemoglobin, serum albumin, total cholesterol, insulin-like growth factor 1 (IGF-1), thyroxine and insulin in GR cattle were significantly lower than those in controls. GR cattle also excreted higher levels of GH. We used three GR and three control cattle for a microarray analysis in the liver and found that 279 gene expressions were significantly different. However,gene expressions related to the GH-IGF-1 axis, such as the GH receptor and IGF-1,were not significantlydifferent from those of controls. Immune-related gene expressions were significantly lower. To clarify these gene expression levels, peripheral lymphocytes were used for real-time RT-PCR. The expression rates of genes that were significantly lower in the liver, such as chemokine ligand 8, interferon gamma receptor 1 and immunoglobulin light chain VJ region were also significantly lower in three GR cattle than those in the three control cattle. These results suggest that the cause of growth retardation in the present study was due to other factors, not abnormal gene expressions of factors related to the GH-IGF-1 axis in the liver, and that GR cattle were susceptible to infectious disease.